Abstract
Background: Early growth response 1 (egr1) is a transcription factor (TF) for controlling the differentiation of Parvalbumin (Parv) -expressing amacrine cells (ACs) in zebrafish. However, the downstream factors of this process have not been identified. The purpose of this study was to investigate the role of p35, a neuronal-specific activator of cyclin-dependent kinase 5 (Cdk5) and a known in vitro target of egr1, in the differentiation of these ACs. Results: In the p35-knockdown retinas, Parv+ but not islet1+ ACs were specifically reduced. This phenotype was highly similar to that in the Egr1-knockdown retinas. Furthermore, p35 expression was reduced in the Egr1-knockdown retinas, particularly in the AC region; while egr1 was only modestly reduced in this region in the p35-knockdown retinas. Conclusions: p35 likely acts downstream of egr1 to control the differentiation of Parv+ ACs. Developmental Dynamics 243:315-323, 2014.
Original language | English (US) |
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Pages (from-to) | 315-323 |
Number of pages | 9 |
Journal | Developmental Dynamics |
Volume | 243 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2014 |
Externally published | Yes |
Keywords
- Amacrine cells
- Retinal development
- Smarca4
- Zebrafish
- cdk5
- cdk5r1b
- egr1
- p35
ASJC Scopus subject areas
- Developmental Biology