P19 embryonic carcinoma cell line: A model to study gene-environment interactions

Joseph Bressler, Cliona O'Driscoll, Cathleen Marshall, Walter Kaufmann

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In studies on pluripotency and differentiation, teratocarcinoma cell lines have been used as alternatives to mouse embryonic stem cell lines. Teratocarcinoma cell lines cost less to maintain and they are easier to genetically manipulate. Their disadvantage, of course, is being derived from a tumor, which prevents their use in certain types of studies such as in implantation and proliferation. The P19 cell line is one of the better studied teratocarcinoma cell lines, being first introduced in 1982. The cells express the same transcription factors for pluripotency maintenance as embryonic stem cells but do not require specialized media or feeder cells. Dimethylsulfoxide induces P19 cells to smooth muscle cells and beating cardiomyocytes, whereas, retinoic acid causes P19 cells to differentiate into functional neurons complete with synapses with specific neurotransmitter phenotypes. Excellent transfection efficiencies can be achieved with the less expensive calcium phosphate method though the more expensive commercially developed reagents also work well. Consequently, transient expression of genes or knocking down gene expression with siRNA is straightforward. Furthermore, establishing stable cell lines with these changes in gene expression is also quite feasible. The procedures for taking advantage of these properties of P19 cells in studies on differentiation and toxicology will be discussed in this chapter.

Original languageEnglish (US)
Title of host publicationCell Culture Techniques
EditorsMichael Aschner, Cristina Sunol, Anna Bal-Price
Pages223-240
Number of pages18
DOIs
StatePublished - Mar 18 2011

Publication series

NameNeuromethods
Volume56
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

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Keywords

  • Differentiation
  • Muscle
  • Neurons
  • P19 cells
  • Pluripotency

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Cite this

Bressler, J., O'Driscoll, C., Marshall, C., & Kaufmann, W. (2011). P19 embryonic carcinoma cell line: A model to study gene-environment interactions. In M. Aschner, C. Sunol, & A. Bal-Price (Eds.), Cell Culture Techniques (pp. 223-240). (Neuromethods; Vol. 56). https://doi.org/10.1007/978-1-61779-077-5_10