P-glycoproteins encoded by mdr 1b in murine gravid uterus and multidrug resistant tumor cell lines are differentially glycosylated

Lee M. Greenberger; James M. Croop; Susan Band Horwitz; Robert J. Arceci

doi:10.1016/0014-5793(89)81586-8

P-glycoproteins encoded by mdr 1b in murine gravid uterus and multidrug resistant tumor cell lines are differentially glycosylated

Lee M. Greenberger, James M. Croop, Susan Band Horwitz, Robert J. Arceci

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

There are 3 members of the multidrug-resitance gene family expressed in mouse. Only one of these, mdr lb, and its gene product P-glycoprotein are induced to high levels in the mouse endometrium during pregnancy. It is shown here that P-glycoprotein in the gravid uterus is significantly larger (M_r 155000) compared to P-glycoprotein encoded by mdr lb in a murine multidrug-resistant cell line (M_r 140000). However, both species co-migrate after enzymatic removal of N-linked sugars (M_r 125000). These results demonstrate that differential glycosylation of the mdr lb gene product contributes to molecular heterogeneity found in P-glycoprotein from normal and multidrug-resistant cells.

Original language	English (US)
Pages (from-to)	419-421
Number of pages	3
Journal	FEBS Letters
Volume	257
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(89)81586-8
State	Published - Nov 6 1989
Externally published	Yes

Keywords

Chemotherapy
Glycosylation
Mouse endometrium
Multidrug resistance
P-glycoprotein
Pregnancy

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(89)81586-8

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title = "P-glycoproteins encoded by mdr 1b in murine gravid uterus and multidrug resistant tumor cell lines are differentially glycosylated",

abstract = "There are 3 members of the multidrug-resitance gene family expressed in mouse. Only one of these, mdr lb, and its gene product P-glycoprotein are induced to high levels in the mouse endometrium during pregnancy. It is shown here that P-glycoprotein in the gravid uterus is significantly larger (Mr 155000) compared to P-glycoprotein encoded by mdr lb in a murine multidrug-resistant cell line (Mr 140000). However, both species co-migrate after enzymatic removal of N-linked sugars (Mr 125000). These results demonstrate that differential glycosylation of the mdr lb gene product contributes to molecular heterogeneity found in P-glycoprotein from normal and multidrug-resistant cells.",

keywords = "Chemotherapy, Glycosylation, Mouse endometrium, Multidrug resistance, P-glycoprotein, Pregnancy",

author = "Greenberger, {Lee M.} and Croop, {James M.} and Horwitz, {Susan Band} and Arceci, {Robert J.}",

note = "Funding Information: Acknowledgements: We thank StephanieG oei DePinho for her ex-cellenta ssistanceT. his work was supportedb y Public Health Service grants from the National Instituteso f Health (R.J.A., lGM38156; J.M.C., lK08CA01227,C A48162;S .B.H., CA39821).L .M.G. is a LeukemiaS ocietyo f America SpecialF ellow.",

year = "1989",

month = nov,

day = "6",

doi = "10.1016/0014-5793(89)81586-8",

language = "English (US)",

volume = "257",

pages = "419--421",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

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T1 - P-glycoproteins encoded by mdr 1b in murine gravid uterus and multidrug resistant tumor cell lines are differentially glycosylated

AU - Greenberger, Lee M.

AU - Croop, James M.

AU - Horwitz, Susan Band

AU - Arceci, Robert J.

N1 - Funding Information: Acknowledgements: We thank StephanieG oei DePinho for her ex-cellenta ssistanceT. his work was supportedb y Public Health Service grants from the National Instituteso f Health (R.J.A., lGM38156; J.M.C., lK08CA01227,C A48162;S .B.H., CA39821).L .M.G. is a LeukemiaS ocietyo f America SpecialF ellow.

PY - 1989/11/6

Y1 - 1989/11/6

N2 - There are 3 members of the multidrug-resitance gene family expressed in mouse. Only one of these, mdr lb, and its gene product P-glycoprotein are induced to high levels in the mouse endometrium during pregnancy. It is shown here that P-glycoprotein in the gravid uterus is significantly larger (Mr 155000) compared to P-glycoprotein encoded by mdr lb in a murine multidrug-resistant cell line (Mr 140000). However, both species co-migrate after enzymatic removal of N-linked sugars (Mr 125000). These results demonstrate that differential glycosylation of the mdr lb gene product contributes to molecular heterogeneity found in P-glycoprotein from normal and multidrug-resistant cells.

AB - There are 3 members of the multidrug-resitance gene family expressed in mouse. Only one of these, mdr lb, and its gene product P-glycoprotein are induced to high levels in the mouse endometrium during pregnancy. It is shown here that P-glycoprotein in the gravid uterus is significantly larger (Mr 155000) compared to P-glycoprotein encoded by mdr lb in a murine multidrug-resistant cell line (Mr 140000). However, both species co-migrate after enzymatic removal of N-linked sugars (Mr 125000). These results demonstrate that differential glycosylation of the mdr lb gene product contributes to molecular heterogeneity found in P-glycoprotein from normal and multidrug-resistant cells.

KW - Chemotherapy

KW - Glycosylation

KW - Mouse endometrium

KW - Multidrug resistance

KW - P-glycoprotein

KW - Pregnancy

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DO - 10.1016/0014-5793(89)81586-8

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AN - SCOPUS:0024787804

SN - 0014-5793

VL - 257

SP - 419

EP - 421

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

P-glycoproteins encoded by mdr 1b in murine gravid uterus and multidrug resistant tumor cell lines are differentially glycosylated

Abstract

Keywords

ASJC Scopus subject areas

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