Ozone, but not nitrogen dioxide, exposure decreases glutathione peroxidases in epithelial lining fluid of human lung

Nelly E. Avissar, Christina K. Reed, Christopher Cox, Mark W. Frampton, Jacob N. Finkelstein

Research output: Contribution to journalArticle

Abstract

Antioxidants, such as glutathione peroxidases (GPxs), in epithelial lining fluid (ELF) protect against health effects of oxidant pollutants, which includes O3 or NO2. We hypothesized that GPxs concentration in ELF is responsive to O3 or NO2 exposure. Subjects underwent two 4-h exposures to O3 (0.22 ppm) and one to air. In another experiment, subjects underwent 3-h exposures to air and NO2 (0.6 and 1.5 ppm). Bronchoalveolar lavage (BAL) was performed immediately or 18 h after O3 exposure and 3.5 h after each NO2 exposure. GPx activity and extracellular GPx (eGPx) protein concentrations were determined in ELF, and their relationships to markers of lung function, inflammation, and epithelial permeability were examined. Although the total amounts were not changed, basal (air) GPx activity (223.6 ± 24.4 mU/ml), basal eGPx protein concentration (2.62 ± 0.25 μg/ml), and basal ELF dilution factor (152.3 ± 8.4) decreased 40% immediately after O3 exposure and remained 30% decreased 18 h after exposure (p = 0.0001). No effect of NO2 exposure on GPxs concentration was detected. There was an inverse correlation between baseline ELF eGPx protein concentration and the change in PMN 18 h after O3 exposure (p = 0.04). Thus, O3, a strong oxidant, decreases both GPx activity and eGPx protein in ELF, whereas NO2, a weaker oxidant, does not. eGPx in ELF may protect against O3-induced airway inflammation.

Original languageEnglish (US)
Pages (from-to)1342-1347
Number of pages6
JournalAmerican journal of respiratory and critical care medicine
Volume162
Issue number4 I
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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