TY - JOUR
T1 - Oxidized low-density lipoprotein is negatively correlated with lecithin-cholesterol acyltransferase activity in type 2 diabetes mellitus
AU - Nakhjavani, Manouchehr
AU - Asgharani, Firouzeh
AU - Khalilzadeh, Omid
AU - Esteghamati, Alireza
AU - Ghaneei, Azam
AU - Morteza, Afsaneh
AU - Anvari, Mehdi
PY - 2011/2
Y1 - 2011/2
N2 - Introduction: It is now believed that the oxidative modification of plasma lipoproteins enhance their atherogenicity in patients with type 2 diabetes. Because a variety of highly reactive lipid peroxidation products can transfer from oxidized low-density lipoprotein (ox-LDL) to high-density lipoprotein-cholesterol, the authors evaluated the association between ox-LDL and lecithin-cholesterol acyltransferase (LCAT) activity, a key enzyme in reverse cholesterol transport and HDL remodeling. Methods: A total of 45 patients with diabetes and 45 age-, sex-and body mass index-matched healthy adult volunteers were enrolled. Fasting blood samples were obtained, and plasma glucose, lipid profile, creatinine, insulin, ox-LDL and LCAT activity were measured. Homeostasis model assessment of insulin resistance was also calculated. Results: Patients with diabetes, compared with healthy participants, had a significantly higher ox-LDL (17.16 ± 3.75 U/L versus 7.93 ± 1.92 U/L, P < 0.001) and lower LCAT activity (73.7 ± 9.1 μmol/L/hr versus 88.7 ± 4.5 μmol/L/hr, P < 0.001). The higher level of LCAT activity completely disappeared after adjustment for ox-LDL. LCAT activity had a significant (P < 0.001) inverse correlation with ox-LDL (r =-0.77) in patients with diabetes and healthy participants (r =-0.75). Conclusion: LCAT activity is significantly decreased in type 2 diabetes. The lower LCAT activity in type 2 diabetes might be through ox-LDL mechanism. Ox-LDL may adversely affect high-density lipoprotein-cholesterol metabolism by reducing LCAT activity.
AB - Introduction: It is now believed that the oxidative modification of plasma lipoproteins enhance their atherogenicity in patients with type 2 diabetes. Because a variety of highly reactive lipid peroxidation products can transfer from oxidized low-density lipoprotein (ox-LDL) to high-density lipoprotein-cholesterol, the authors evaluated the association between ox-LDL and lecithin-cholesterol acyltransferase (LCAT) activity, a key enzyme in reverse cholesterol transport and HDL remodeling. Methods: A total of 45 patients with diabetes and 45 age-, sex-and body mass index-matched healthy adult volunteers were enrolled. Fasting blood samples were obtained, and plasma glucose, lipid profile, creatinine, insulin, ox-LDL and LCAT activity were measured. Homeostasis model assessment of insulin resistance was also calculated. Results: Patients with diabetes, compared with healthy participants, had a significantly higher ox-LDL (17.16 ± 3.75 U/L versus 7.93 ± 1.92 U/L, P < 0.001) and lower LCAT activity (73.7 ± 9.1 μmol/L/hr versus 88.7 ± 4.5 μmol/L/hr, P < 0.001). The higher level of LCAT activity completely disappeared after adjustment for ox-LDL. LCAT activity had a significant (P < 0.001) inverse correlation with ox-LDL (r =-0.77) in patients with diabetes and healthy participants (r =-0.75). Conclusion: LCAT activity is significantly decreased in type 2 diabetes. The lower LCAT activity in type 2 diabetes might be through ox-LDL mechanism. Ox-LDL may adversely affect high-density lipoprotein-cholesterol metabolism by reducing LCAT activity.
KW - Diabetes mellitus
KW - LCAT activity
KW - Ox-LDL
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U2 - 10.1097/MAJ.0b013e3181f3ce10
DO - 10.1097/MAJ.0b013e3181f3ce10
M3 - Article
C2 - 20890173
AN - SCOPUS:79951518173
SN - 0002-9629
VL - 341
SP - 92
EP - 95
JO - American Journal of the Medical Sciences
JF - American Journal of the Medical Sciences
IS - 2
ER -