Oxidative Stress Induced by Cigarette Smoke Extracts in Human Brain Cells (T98G) and Human Brain Microvascular Endothelial Cells (HBMEC) in Mono-and Co-Culture

Ju Hyeong Kim, Myung Haing Cho, Kyung Chul Choi, Kyuhong Lee, Kwang Sik Kim, Soon Mi Shim

Research output: Contribution to journalArticle

Abstract

The objective of the current study was to examine oxidative stress induced by cigarette smoke extract (CSE) or cigarette smoke condensate (CSC) in human brain cells (T98G) and human brain microvascular endothelial cells (HBMEC) in mono-and co-culture systems. Cell viability of T98G cells exposed to CSC (0.05-4 mg/ml) was significantly decreased compared to CSE (0.025-20%). There were no marked differences between quantities of reactive oxygen species (ROS) generation by either CSE (2, 4, and 10%) or CSC (0.2, 0.4, and 0.8 mg/ml) treatment compared to control. However, a significant effect was noted in ROS generation following CSC incubation at 4mg/ml. Cellular integrity of HBMEC decreased to 74 and 64% within 120 h of exposure at the IC50 value of CSE and CSC, respectively. This study suggests that chronic exposure to cigarette smoking might initiate damage to the blood-brain barrier.

Original languageEnglish (US)
Pages (from-to)1019-1027
Number of pages9
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume78
Issue number15
DOIs
StatePublished - Aug 3 2015

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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