Ovine lentivirus expression and disease: Virus replication, but not entry, is restricted to macrophages of specific tissues

S. J. Brodie, L. D. Pearson, M. C. Zink, H. M. Bickle, B. C. Anderson, K. A. Marcom, J. C. DeMartini

Research output: Contribution to journalArticlepeer-review

Abstract

To better define the relationship between lentivirus infection and lymphoproliferative or inflammatory disease, we studied postmortem specimens of 38 sheep naturally infected with ovine lentivirus (OvLV) and with different clinical manifestations of OvLV-associated disease. Immunohistochemistry, in situ hybridization, and virus isolation were used to localize viral protein, viral RNA, and infectious virus to specific cells and tissues. Viral protein or infectious virus was found in cells morphologically and histochemically compatible with macrophages (Mφs), but only in lung, bone marrow, mammary gland, lymph node, spleen, synovium, brain, and spinal cord, frequently in association with lymphocyte infiltrates. In contrast, viral RNA was found in a variety of cell types, including epithelium, Mφs, and Mφ-like cells, and in a wider range of tissues, with or without OvLV- associated lesions. In summary, these findings suggest that in vivo: 1), OvLV can enter a variety of cell types, 2), productive infection is restricted to cells of Mφ lineage, and 3), cells expressing viral proteins are limited to specific tissues, those associated with OvLV-induced diseases.

Original languageEnglish (US)
Pages (from-to)250-263
Number of pages14
JournalAmerican Journal of Pathology
Volume146
Issue number1
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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