Overview of RAW264.7 for osteoclastogensis study: Phenotype and stimuli

Lingbo Kong, Wanli Smith, Dingjun Hao

Research output: Contribution to journalReview articlepeer-review


Bone homeostasis is preserved by the balance of maintaining between the activity of osteogenesis and osteoclastogenesis. However, investigations for the osteoclastogenesis were hampered by considerable difficulties associated with isolating and culturing osteoclast in vivo. As the alternative, stimuli-induced osteoclasts formation from RAW264.7 cells (RAW-OCs) have gain its importance for extensively osteoclastogenic study of bone diseases, such as rheumatoid arthritis, osteoporosis, osteolysis and periodontitis. However, considering the RAW-OCs have not yet been well-characterized and RAW264.7 cells are polymorphic because of a diverse phenotype of the individual cells comprising this cell linage, and different fate associated with various stimuli contributions. Thus, in present study, we provide an overview for current knowledge of the phenotype of RAW264.7 cells, as well as the current understanding of the complicated interactions between various stimuli and RAW-OCs in the light of the recent progress.

Original languageEnglish (US)
Pages (from-to)3077-3087
Number of pages11
JournalJournal of Cellular and Molecular Medicine
Issue number5
StatePublished - May 2019


  • LPS
  • RAW264.7
  • TNF-a
  • bone marrow macrophage
  • osteoclast
  • phenotype

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology


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