Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer

Deepthi Telikicherla, Jagadeesha Maharudraiah, Harsh Pawar, Arivusudar Marimuthu, Manoj Kumar Kashyap, Y. L. Ramachandra, Juan Carlos Roa, Akhilesh Pandey

Research output: Contribution to journalArticle

Abstract

Gene expression profiling studies on breast cancer have generated catalogs of differentially expressed genes. However, many of these genes have not been investigated for their expression at the protein level. It is possible to systematically evaluate such genes in a high-throughput fashion for their overexpression at the protein level using breast cancer tissue microarrays. Our strategy involved integration of information from publicly available repositories of gene expression to prepare a list of genes upregulated at the mRNA level in breast cancer followed by curation of the published literature to identify those genes that were not tested for overexpression at the protein level. We identified Kinesin Associated Protein 3 (KIFAP3) as one such molecule for further validation at the protein level. Immunohistochemical labeling of KIFAP3 using breast cancer tissue microarrays revealed overexpression of KIFAP3 protein in 84% (240/285) of breast cancers indicating the utility of our integrated approach of combining computational analysis with experimental biology.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalJournal of Proteomics and Bioinformatics
Volume5
Issue number5
DOIs
StatePublished - May 2012

Fingerprint

Kinesin
Breast Neoplasms
Proteins
Genes
Microarrays
Gene expression
Tissue
Gene Expression Profiling
Labeling
Throughput
Gene Expression
Messenger RNA
Molecules

Keywords

  • Breast neoplasms
  • Immunohistochemistry
  • Kap3
  • Smap
  • Smggds associated protein

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Computer Science Applications

Cite this

Telikicherla, D., Maharudraiah, J., Pawar, H., Marimuthu, A., Kashyap, M. K., Ramachandra, Y. L., ... Pandey, A. (2012). Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer. Journal of Proteomics and Bioinformatics, 5(5), 122-126. https://doi.org/10.4172/jpb.1000223

Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer. / Telikicherla, Deepthi; Maharudraiah, Jagadeesha; Pawar, Harsh; Marimuthu, Arivusudar; Kashyap, Manoj Kumar; Ramachandra, Y. L.; Roa, Juan Carlos; Pandey, Akhilesh.

In: Journal of Proteomics and Bioinformatics, Vol. 5, No. 5, 05.2012, p. 122-126.

Research output: Contribution to journalArticle

Telikicherla, D, Maharudraiah, J, Pawar, H, Marimuthu, A, Kashyap, MK, Ramachandra, YL, Roa, JC & Pandey, A 2012, 'Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer', Journal of Proteomics and Bioinformatics, vol. 5, no. 5, pp. 122-126. https://doi.org/10.4172/jpb.1000223
Telikicherla D, Maharudraiah J, Pawar H, Marimuthu A, Kashyap MK, Ramachandra YL et al. Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer. Journal of Proteomics and Bioinformatics. 2012 May;5(5):122-126. https://doi.org/10.4172/jpb.1000223
Telikicherla, Deepthi ; Maharudraiah, Jagadeesha ; Pawar, Harsh ; Marimuthu, Arivusudar ; Kashyap, Manoj Kumar ; Ramachandra, Y. L. ; Roa, Juan Carlos ; Pandey, Akhilesh. / Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer. In: Journal of Proteomics and Bioinformatics. 2012 ; Vol. 5, No. 5. pp. 122-126.
@article{f542f7583b764ad7a03cc0c616589733,
title = "Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer",
abstract = "Gene expression profiling studies on breast cancer have generated catalogs of differentially expressed genes. However, many of these genes have not been investigated for their expression at the protein level. It is possible to systematically evaluate such genes in a high-throughput fashion for their overexpression at the protein level using breast cancer tissue microarrays. Our strategy involved integration of information from publicly available repositories of gene expression to prepare a list of genes upregulated at the mRNA level in breast cancer followed by curation of the published literature to identify those genes that were not tested for overexpression at the protein level. We identified Kinesin Associated Protein 3 (KIFAP3) as one such molecule for further validation at the protein level. Immunohistochemical labeling of KIFAP3 using breast cancer tissue microarrays revealed overexpression of KIFAP3 protein in 84{\%} (240/285) of breast cancers indicating the utility of our integrated approach of combining computational analysis with experimental biology.",
keywords = "Breast neoplasms, Immunohistochemistry, Kap3, Smap, Smggds associated protein",
author = "Deepthi Telikicherla and Jagadeesha Maharudraiah and Harsh Pawar and Arivusudar Marimuthu and Kashyap, {Manoj Kumar} and Ramachandra, {Y. L.} and Roa, {Juan Carlos} and Akhilesh Pandey",
year = "2012",
month = "5",
doi = "10.4172/jpb.1000223",
language = "English (US)",
volume = "5",
pages = "122--126",
journal = "Journal of Proteomics and Bioinformatics",
issn = "0974-276X",
publisher = "Omics Publishing Group",
number = "5",

}

TY - JOUR

T1 - Overexpression of kinesin associated protein 3 (KIFAP3) in breast cancer

AU - Telikicherla, Deepthi

AU - Maharudraiah, Jagadeesha

AU - Pawar, Harsh

AU - Marimuthu, Arivusudar

AU - Kashyap, Manoj Kumar

AU - Ramachandra, Y. L.

AU - Roa, Juan Carlos

AU - Pandey, Akhilesh

PY - 2012/5

Y1 - 2012/5

N2 - Gene expression profiling studies on breast cancer have generated catalogs of differentially expressed genes. However, many of these genes have not been investigated for their expression at the protein level. It is possible to systematically evaluate such genes in a high-throughput fashion for their overexpression at the protein level using breast cancer tissue microarrays. Our strategy involved integration of information from publicly available repositories of gene expression to prepare a list of genes upregulated at the mRNA level in breast cancer followed by curation of the published literature to identify those genes that were not tested for overexpression at the protein level. We identified Kinesin Associated Protein 3 (KIFAP3) as one such molecule for further validation at the protein level. Immunohistochemical labeling of KIFAP3 using breast cancer tissue microarrays revealed overexpression of KIFAP3 protein in 84% (240/285) of breast cancers indicating the utility of our integrated approach of combining computational analysis with experimental biology.

AB - Gene expression profiling studies on breast cancer have generated catalogs of differentially expressed genes. However, many of these genes have not been investigated for their expression at the protein level. It is possible to systematically evaluate such genes in a high-throughput fashion for their overexpression at the protein level using breast cancer tissue microarrays. Our strategy involved integration of information from publicly available repositories of gene expression to prepare a list of genes upregulated at the mRNA level in breast cancer followed by curation of the published literature to identify those genes that were not tested for overexpression at the protein level. We identified Kinesin Associated Protein 3 (KIFAP3) as one such molecule for further validation at the protein level. Immunohistochemical labeling of KIFAP3 using breast cancer tissue microarrays revealed overexpression of KIFAP3 protein in 84% (240/285) of breast cancers indicating the utility of our integrated approach of combining computational analysis with experimental biology.

KW - Breast neoplasms

KW - Immunohistochemistry

KW - Kap3

KW - Smap

KW - Smggds associated protein

UR - http://www.scopus.com/inward/record.url?scp=84866545926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866545926&partnerID=8YFLogxK

U2 - 10.4172/jpb.1000223

DO - 10.4172/jpb.1000223

M3 - Article

C2 - 26843789

AN - SCOPUS:84866545926

VL - 5

SP - 122

EP - 126

JO - Journal of Proteomics and Bioinformatics

JF - Journal of Proteomics and Bioinformatics

SN - 0974-276X

IS - 5

ER -