Overexpression of CDC91L1 (PIG-U) in bladder urothelial cell carcinoma: Correlation with clinical variables and prognostic significance

Yi Jun Shen, Ding Wei Ye, Xu Dong Yao, Barry Trink, Xiao Yan Zhou, Shi Lin Zhang, Bo Dai, Hai Liang Zhang, Yao Zhu, Zhongmin Guo, Guojun Wu, Jatin Nagpal

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: To investigate cell division cycle 91-like 1 (CDC91L1; also called phosphatidylinositol glycan class U, PIG-U) expression in bladder cancer at both the mRNA and protein levels, and to study its clinical and prognostic significance, as CDC91L1 was recently identified as a new oncogene in human bladder cancer and its role in the biological behaviour of bladder cancer is largely unknown. PATIENTS AND METHODS: In all, 73 bladder tumours and 14 samples of normal bladder urothelium were studied by reverse-transcription polymerase chain reaction (PCR), real-time quantitative PCR and immunohistochemistry. RESULTS: The normalized CDC91L1 mRNA copy number in tumours was significantly greater than in normal controls (P < 0.05). There was overexpression of CDC91L1 mRNA in 30.1% (22/73) of the bladder tumours compared with the normal urothelium. At the protein level, 75.3% (55/73) of the bladder tumours and two of 14 of the normal urothelium had high expression of CDC91L1 protein, which is statistically significant (P < 0.001). The correlation between CDC91L1 protein and tumour grade, and muscle invasion of tumour was significant (both P < 0.05). In addition to tumour extent and tumour grade, CDC91L1 protein was an independent predictor of recurrence for superficial bladder cancer and had a trend to predict tumour progression. CONCLUSIONS: CDC91L1 (PIG-U) plays a role in the development of bladder urothelial cell carcinoma. CDC91L1 protein might be a potential biomarker for prediction of recurrence and a therapeutic target in bladder cancer.

Original languageEnglish (US)
Pages (from-to)113-119
Number of pages7
JournalBJU International
Volume101
Issue number1
DOIs
StatePublished - Jan 2008

Keywords

  • Bladder neoplasms
  • Glycosylphosphatidylinositols
  • Prognosis
  • Progression
  • Quantitative real-time PCR
  • Recurrence

ASJC Scopus subject areas

  • Urology

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