TY - JOUR
T1 - Overexpression of c-met in the early stage of pancreatic carcinogenesis; altered expression is not sufficient for progression from chronic pancreatitis to pancreatic cancer
AU - Yu, Jun
AU - Ohuchida, Kenoki
AU - Mizumoto, Kazuhiro
AU - Ishikawa, Nami
AU - Ogura, Yasuhiro
AU - Yamada, Daisuke
AU - Egami, Takuya
AU - Fujita, Hayato
AU - Ohashi, Seiji
AU - Nagai, Eishi
AU - Tanaka, Masao
PY - 2006/6/28
Y1 - 2006/6/28
N2 - Aim: To investigate c-met expression during early pancreatic carcinogenesis. Methods: We used 46 bulk tissues and 36 micro-dissected samples, including normal pancreas, chronic pancreatitis, and pancreatic cancer, for quantitative real-time reverse transcription-polymerase chain reaction. Results: In bulk tissue analyses, pancreatic cancer tissues expressed significantly higher levels of c-met than did chronic pancreatitis and normal pancreas tissues. c-met levels did not differ between chronic pancreatitis and normal pancreas tissues. In microdissection-based analyses, c-met was expressed at higher levels in microdissected pancreatic cancer cells and pancreatitis-affected epithelial cells than in normal ductal epithelial cells (both, P < 0.01). Interestingly, pancreatitis-affected epithelial cells expressed levels of c-met similar to those of pancreatic cancer cells. Conclusion: Overexpression of c-met occurs during the early stage of pancreatic carcinogenesis, and a single alteration of c-met expression is not sufficient for progression of chronic pancreatitis-affected epithelial cells to pancreatic cancer cells.
AB - Aim: To investigate c-met expression during early pancreatic carcinogenesis. Methods: We used 46 bulk tissues and 36 micro-dissected samples, including normal pancreas, chronic pancreatitis, and pancreatic cancer, for quantitative real-time reverse transcription-polymerase chain reaction. Results: In bulk tissue analyses, pancreatic cancer tissues expressed significantly higher levels of c-met than did chronic pancreatitis and normal pancreas tissues. c-met levels did not differ between chronic pancreatitis and normal pancreas tissues. In microdissection-based analyses, c-met was expressed at higher levels in microdissected pancreatic cancer cells and pancreatitis-affected epithelial cells than in normal ductal epithelial cells (both, P < 0.01). Interestingly, pancreatitis-affected epithelial cells expressed levels of c-met similar to those of pancreatic cancer cells. Conclusion: Overexpression of c-met occurs during the early stage of pancreatic carcinogenesis, and a single alteration of c-met expression is not sufficient for progression of chronic pancreatitis-affected epithelial cells to pancreatic cancer cells.
KW - Chronic pancreatitis
KW - Pancreatic cancer
KW - Pancreatic carcinogenesis
KW - c-met
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UR - http://www.scopus.com/inward/citedby.url?scp=33745853820&partnerID=8YFLogxK
U2 - 10.3748/wjg.v12.i24.3878
DO - 10.3748/wjg.v12.i24.3878
M3 - Article
C2 - 16804974
AN - SCOPUS:33745853820
SN - 1007-9327
VL - 12
SP - 3878
EP - 3882
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 24
ER -