Overexpression of a dominant-negative signal transducer and activator of transcription 3 variant in tumor cells leads to production of soluble factors that induce apoptosis and cell cycle arrest

G. Niu, K. H. Shain, M. Huang, Rajani Ravi, Atul Bedi, W. S. Dalton, R. Jove, H. Yu

Research output: Contribution to journalArticle

Abstract

Gene therapy of B16 tumors with a dominant-negative signal transducer and activator of transcription (Stat3) variant, designated Stat3β, results in inhibition of tumor growth and tumor regression. Although only 10-15% of the tumor cells are transfected in vivo, the Stat3β-induced antitumor effect is associated with massive apoptosis of B16 tumor cells, indicative of a potent bystander effect. Here, we provide evidence that blocking Stat3 signaling in B16 cells results in release of soluble factors that are capable of inducing apoptosis and cell cycle arrest of nontransfected B16 cells. RNase protection assays using multi-template probes specific for key physiological regulators of apoptosis reveal that overexpression of Stat3β in B16 tumor cells induces the expression of the apoptotic effector, tumor necrosis factor-related apoptosis-inducing ligand. These in vitro results suggest that the observed in vivo bystander effect leading to tumor cell growth inhibition is mediated, at least in part, by soluble factors produced as a result of overexpression of Stat3β in tumor cells.

Original languageEnglish (US)
Pages (from-to)3276-3280
Number of pages5
JournalCancer Research
Volume61
Issue number8
StatePublished - Apr 15 2001

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STAT3 Transcription Factor
Cell Cycle Checkpoints
Apoptosis
Neoplasms
Bystander Effect
Apoptosis Inducing Factor
Ribonucleases
Growth
Transducers
Genetic Therapy
Tumor Necrosis Factor-alpha
Ligands

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Overexpression of a dominant-negative signal transducer and activator of transcription 3 variant in tumor cells leads to production of soluble factors that induce apoptosis and cell cycle arrest. / Niu, G.; Shain, K. H.; Huang, M.; Ravi, Rajani; Bedi, Atul; Dalton, W. S.; Jove, R.; Yu, H.

In: Cancer Research, Vol. 61, No. 8, 15.04.2001, p. 3276-3280.

Research output: Contribution to journalArticle

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AU - Shain, K. H.

AU - Huang, M.

AU - Ravi, Rajani

AU - Bedi, Atul

AU - Dalton, W. S.

AU - Jove, R.

AU - Yu, H.

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AB - Gene therapy of B16 tumors with a dominant-negative signal transducer and activator of transcription (Stat3) variant, designated Stat3β, results in inhibition of tumor growth and tumor regression. Although only 10-15% of the tumor cells are transfected in vivo, the Stat3β-induced antitumor effect is associated with massive apoptosis of B16 tumor cells, indicative of a potent bystander effect. Here, we provide evidence that blocking Stat3 signaling in B16 cells results in release of soluble factors that are capable of inducing apoptosis and cell cycle arrest of nontransfected B16 cells. RNase protection assays using multi-template probes specific for key physiological regulators of apoptosis reveal that overexpression of Stat3β in B16 tumor cells induces the expression of the apoptotic effector, tumor necrosis factor-related apoptosis-inducing ligand. These in vitro results suggest that the observed in vivo bystander effect leading to tumor cell growth inhibition is mediated, at least in part, by soluble factors produced as a result of overexpression of Stat3β in tumor cells.

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