TY - JOUR
T1 - Overexpression of a chromatin remodeling factor, RSF-1/HBXAP, correlates with aggressive oral squamous cell carcinoma
AU - Fang, Fu Min
AU - Li, Chien Feng
AU - Huang, Hsuan Ying
AU - Lai, Ming Tsong
AU - Chen, Chih Mei
AU - Chiu, I. Wen
AU - Wang, Tian Li
AU - Tsai, Fuu Jen
AU - Shih, Ie Ming
AU - Sheu, Jim Jinn Chyuan
N1 - Funding Information:
This study was supported in part by funds from the National Institutes of Health/National Cancer Institute grant RO1CA129080 (I.M.S.), the National Science Council , Taiwan grant NSC96-2321-B-182A-001-MY2 (C.F.L.) and grant NSC98-2320-B-039-033-MY3 (J.J.S.), the Department of Health, Taiwan grant DOH99-TD-C-111-004 (J.J.S.), and the China Medical University , Taiwan grant CMU97-280 (J.J.S.).
PY - 2011/5
Y1 - 2011/5
N2 - RSF-1, also known as hepatitis B X-antigen associated protein (HBXAP), is a subunit of an ISWI chromatin remodeling complex, remodeling and spacing factor (RSF). Recent studies have provided new evidence that chromatin remodeling participates in the pathogenesis of neoplastic diseases by altering cell cycle regulation and gene expression. In this report, we studied the biological roles of RSF-1 in oral squamous cell carcinoma (OSCC), a highly invasive neoplastic disease. Based on IHC and quantitative real-time PCR, we demonstrated that RSF-1 expression could be detected in the majority of OSCC cases, and the levels were significantly higher in OSCC cells than in their normal counterparts. Moreover, expression levels of RSF-1 significantly correlated with the presence of angiolymphatic invasion, abnormal mitoses, metastasis, tumor recurrence, and advanced stage disease at presentation. Univariate and multivariate analyses showed a significant association of RSF-1 overexpression and worse overall survival in OSCC patients. RSF-1 knockdown remarkably decreased cellular proliferation and induced apoptosis in OSCC cells with high RSF-1 expression levels, but not in those without. Taken together, our results suggest that RSF-1 up-regulation is associated with several clinicopathological features of disease aggressiveness in OSCC patients, and RSF-1 plays an important role in maintaining cellular growth and survival in OSCC.
AB - RSF-1, also known as hepatitis B X-antigen associated protein (HBXAP), is a subunit of an ISWI chromatin remodeling complex, remodeling and spacing factor (RSF). Recent studies have provided new evidence that chromatin remodeling participates in the pathogenesis of neoplastic diseases by altering cell cycle regulation and gene expression. In this report, we studied the biological roles of RSF-1 in oral squamous cell carcinoma (OSCC), a highly invasive neoplastic disease. Based on IHC and quantitative real-time PCR, we demonstrated that RSF-1 expression could be detected in the majority of OSCC cases, and the levels were significantly higher in OSCC cells than in their normal counterparts. Moreover, expression levels of RSF-1 significantly correlated with the presence of angiolymphatic invasion, abnormal mitoses, metastasis, tumor recurrence, and advanced stage disease at presentation. Univariate and multivariate analyses showed a significant association of RSF-1 overexpression and worse overall survival in OSCC patients. RSF-1 knockdown remarkably decreased cellular proliferation and induced apoptosis in OSCC cells with high RSF-1 expression levels, but not in those without. Taken together, our results suggest that RSF-1 up-regulation is associated with several clinicopathological features of disease aggressiveness in OSCC patients, and RSF-1 plays an important role in maintaining cellular growth and survival in OSCC.
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U2 - 10.1016/j.ajpath.2011.01.043
DO - 10.1016/j.ajpath.2011.01.043
M3 - Article
C2 - 21514451
AN - SCOPUS:79959228633
SN - 0002-9440
VL - 178
SP - 2407
EP - 2415
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 5
ER -