Over-expression of p27kip1 affects cell cycle of hepatocellular carcinoma cell lines

Jie Wu, Ying Ying Sang, Jing Tao, Ying Ming Wang, Chao Qun Wu, Ke Jing Deng, Shou Yi Qiao

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To study the effects of P27 protein, encoded by p27 kip1, a crucial tumor suppression gene, on hepatocellular carcinoma(HCC) cell lines HepG2 and Hep3B, when it is over-expressed. Methods: We constructed mutant P27-expressing plasmids, pEYFP-P27 (S10A), pEYFP-P27 (T157A) and pEYFP-P27(T187A), by site-mutation technique from pEYFP-P27(WT) which encodes wild type P27 protein. HCC cell lines, HepG2 and Hep3B, were transfected with pEYFP-P27(WT) as well as pEYFPK-P27(mutant). Innate and over-expressed P27 protein were examined using Western blot, and their effects on cell cycle were analyzed by FACS, respectively. Results: It was found that overexpression of all P27 fusion proteins could strengthen G1 phase arrest in HepG2 and Hep3B cells; T157A mutant beared stronger activity of G0/G1 arrest than wild type or other mutant in HepG2, while in Hep3B no remarkable differences were detected between 3 mutants and wild type P27. Conclusions: Overexpression of P27 protein can remarkably strengthen G0/G1 phase arrest in HCC cell lines.

Original languageEnglish (US)
Pages (from-to)323-328
Number of pages6
JournalFudan University Journal of Medical Sciences
Volume34
Issue number3
StatePublished - May 1 2007
Externally publishedYes

Keywords

  • Cell cycle
  • Hepatocellular carcinoma cell lines
  • P27 protein

ASJC Scopus subject areas

  • Medicine(all)

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