Ovarian cancer-associated ascites demonstrates altered immune environment: Implications for antitumor immunity

Robert L. Giuntoli, Tonya J. Webb, Alessia Zoso, Ophelia Rogers, Teresa P. Diaz-Montes, Robert E. Bristow, Mathias Oelke

Research output: Contribution to journalArticle

Abstract

Background: To identify immunosuppressive elements present in ovarian cancer associated ascites. Patients and Methods: Ascites and plasma were obtained from ovarian, primary peritoneal or fallopian tube cancer patients. Surface markers were identified by fluorescence-activated cell sorting (FACS). Cytokine and chemokine concentrations were measured with LINCOplex microarrays. Antigen-specific T-lymphocytes from ascites and plasma were expanded with artificial antigen-presenting cells (aAPC). Cell-mediated immune response was assessed with chromium release assays. Results: Samples were collected from 37 patients with advanced ovarian cancer. FACS was performed on 27 ascites specimens. A low CD4/CD8 ratio (

Original languageEnglish (US)
Pages (from-to)2875-2884
Number of pages10
JournalAnticancer Research
Volume29
Issue number8
StatePublished - Aug 2009

Fingerprint

Ascites
Ovarian Neoplasms
Immunity
Flow Cytometry
Fallopian Tube Neoplasms
CD4-CD8 Ratio
Chromium
Antigen-Presenting Cells
Immunosuppressive Agents
Chemokines
Cytokines
T-Lymphocytes
Antigens

Keywords

  • Ascites
  • Cell mediated immunity
  • Chemokines
  • Cytokines
  • Ovarian cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Giuntoli, R. L., Webb, T. J., Zoso, A., Rogers, O., Diaz-Montes, T. P., Bristow, R. E., & Oelke, M. (2009). Ovarian cancer-associated ascites demonstrates altered immune environment: Implications for antitumor immunity. Anticancer Research, 29(8), 2875-2884.

Ovarian cancer-associated ascites demonstrates altered immune environment : Implications for antitumor immunity. / Giuntoli, Robert L.; Webb, Tonya J.; Zoso, Alessia; Rogers, Ophelia; Diaz-Montes, Teresa P.; Bristow, Robert E.; Oelke, Mathias.

In: Anticancer Research, Vol. 29, No. 8, 08.2009, p. 2875-2884.

Research output: Contribution to journalArticle

Giuntoli, RL, Webb, TJ, Zoso, A, Rogers, O, Diaz-Montes, TP, Bristow, RE & Oelke, M 2009, 'Ovarian cancer-associated ascites demonstrates altered immune environment: Implications for antitumor immunity', Anticancer Research, vol. 29, no. 8, pp. 2875-2884.
Giuntoli RL, Webb TJ, Zoso A, Rogers O, Diaz-Montes TP, Bristow RE et al. Ovarian cancer-associated ascites demonstrates altered immune environment: Implications for antitumor immunity. Anticancer Research. 2009 Aug;29(8):2875-2884.
Giuntoli, Robert L. ; Webb, Tonya J. ; Zoso, Alessia ; Rogers, Ophelia ; Diaz-Montes, Teresa P. ; Bristow, Robert E. ; Oelke, Mathias. / Ovarian cancer-associated ascites demonstrates altered immune environment : Implications for antitumor immunity. In: Anticancer Research. 2009 ; Vol. 29, No. 8. pp. 2875-2884.
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