Ovarian and endometrial endometrioid adenocarcinomas have distinct profiles of microsatellite instability, PTEN expression, and ARID1A expression

Hsien Neng Huang, Ming Chieh Lin, Li Hui Tseng, Ying Cheng Chiang, Liang In Lin, Yu Feng Lin, Hsin Ying Huang, Kuan Ting Kuo

Research output: Contribution to journalArticle

Abstract

Aims: To understand the role of and differences in molecular alterations between endometrial and ovarian endometrioid adenocarcinomas. Methods and results: We investigated the microsatellite status of 26 ovarian endometrioid adenocarcinomas (OVEMs), 42 endometrial endometrioid adenocarcinomas (EMCAs), and 19 concurrent (endometrial and ovarian) endometrioid adenocarcinomas. We evaluated the expression of the mismatch repair proteins, PTEN and ARID1A, and mutations of PTEN, KRAS, CTNNB1, and PIK3CA. High levels of microsatellite instability (MSI-H) were present in one of 26 OVEMs, 12 of 42 EMCAs, and four of 19 concurrent endometrioid adenocarcinomas. Only four of 19 concurrent endometrioid adenocarcinomas showed identical molecular alterations in their endometrial and ovarian components. Loss of ARID1A or loss of PTEN expression, and MSI-H, were more common in EMCAs than OVEMs (P = 0.044, P = 0.004, and P = 0.012, respectively). MSI-H in endometrial endometrioid adenocarcinomas was also related to loss of ARID1A expression (P <0.001). In the cohort of MSI-H endometrioid adenocarcinomas involving the endometrium (n = 16), MSH6-deficient cases showed higher frequencies of CTNNB1 and PIK3CA mutations (P = 0.008 and P = 0.036, respectively), but lower frequencies of KRAS mutation (P = 0.011), than PMS2-deficient cases. Conclusions: The different frequencies of molecular genetic alterations between endometrial endometrioid adenocarcinomas and ovarian endometrioid adenocarcinomas imply that distinct processes may be involved in their tumorigenesis or tumour progression.

Original languageEnglish (US)
Pages (from-to)517-528
Number of pages12
JournalHistopathology
Volume66
Issue number4
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Keywords

  • PTEN
  • ARID1A
  • CTNNB1
  • Endometrial cancer
  • Endometrioid adenocarcinoma
  • Microsatellite instability
  • Mismatch repair proteins
  • Ovarian cancer

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine
  • Medicine(all)

Fingerprint Dive into the research topics of 'Ovarian and endometrial endometrioid adenocarcinomas have distinct profiles of microsatellite instability, PTEN expression, and ARID1A expression'. Together they form a unique fingerprint.

  • Cite this

    Huang, H. N., Lin, M. C., Tseng, L. H., Chiang, Y. C., Lin, L. I., Lin, Y. F., Huang, H. Y., & Kuo, K. T. (2015). Ovarian and endometrial endometrioid adenocarcinomas have distinct profiles of microsatellite instability, PTEN expression, and ARID1A expression. Histopathology, 66(4), 517-528. https://doi.org/10.1111/his.12543