Otitis media, antibiotics, and risk of autism spectrum disorder

Theresa Wimberley, Esben Agerbo, Carsten B. Pedersen, Søren Dalsgaard, Henriette Thisted Horsdal, Preben B. Mortensen, Wesley K. Thompson, Ole Köhler-Forsberg, Robert H. Yolken

Research output: Contribution to journalArticlepeer-review


Otitis media infections and antibiotic treatment have been linked to the risk of developing autism spectrum disorder. Broad-spectrum antibiotics may alter the composition of the gut flora microbiota, which is hypothesized to be involved in the regulation of the immune system. This study examines the interplay among otitis media, antibiotics, and the subsequent risk of developing autism. Based on the entire Danish population, 780,547 children were followed from birth (January 1, 1997 to December 31, 2008) until December 31, 2012. We calculated adjusted hazard ratios and absolute risks of autism with 95% confidence intervals (CIs) related to previous otitis media diagnoses and antibiotic prescriptions redeemed at Danish pharmacies. The absolute risk of autism before age 10 was increased among children with otitis media (1.2% for females and 3.3% for males) and in children who had redeemed an antibiotic prescription (0.6% and 2.7% for females and males) compared to children without a history of otitis media and antibiotics usage (0.4% for females and 1.9% for males). Similarly, we found an increased hazard ratio of autism associated with otitis media (1.83 95% CI 1.71–1.95) and antibiotics usage (1.29 95% CI 1.17–1.43). A history of both otitis media and antibiotic treatment did not further increase the risk of autism. Although the risk of autism was associated with otitis media and treatment with antibiotics, we found little evidence of a synergistic effect between otitis media infections and treatment with antibiotics. Autism Res 2018, 11: 1432–1440.

Original languageEnglish (US)
Pages (from-to)1432-1440
Number of pages9
JournalAutism Research
Issue number10
StatePublished - Oct 2018
Externally publishedYes


  • antibiotics
  • autism spectrum disorder
  • epidemiology
  • otitis media

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Genetics(clinical)


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