Osteogenic potential of embryonic stem cells in tooth sockets

Hyun Ki Kang, Sangho Roh, Gabsang Lee, Seong Doo Hong, Hoin Kang, Byung Moo Min

Research output: Contribution to journalArticle

Abstract

Embryonic stem cells (ESCs) are established from blastocysts and give rise to various types of cells and tissues. In the present study, we assessed the osteogenic potential of ESCs using in vitro culture conditions and in vivo differentiation in tooth sockets. An ESC-derived embryoid body (EB) was formed and subsequently induced to an osteogenic lineage. The differentiated EB cells exhibited increased expression of various osteogenic markers as determined by real-time PCR analysis. Likewise, the differentiated EB-derived cells had enhanced alkaline phosphatase activity and calcium accumulation, as determined by cytochemical methods. For in vivo transplantation, mixtures of ESCs and hydroxyapatite/tricalcium phosphate particles or EBs alone were transplanted into female rat tooth sockets. After 12 weeks, we observed formation of osteogenic structure in the tooth sockets without evidence of teratomas. These data suggest that pluripotent ESCs can serve as an alternative source for the reconstruction of craniofacial structures, as well as for further applications.

Original languageEnglish (US)
Pages (from-to)539-544
Number of pages6
JournalInternational journal of molecular medicine
Volume21
Issue number5
StatePublished - May 1 2008
Externally publishedYes

Keywords

  • Differentiation
  • Embryoid body
  • Embryonic stem cell
  • Osteoid
  • Tooth socket
  • Transplantation

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Kang, H. K., Roh, S., Lee, G., Hong, S. D., Kang, H., & Min, B. M. (2008). Osteogenic potential of embryonic stem cells in tooth sockets. International journal of molecular medicine, 21(5), 539-544.