Osteoclast-like multinucleated giant cells in sinonasal inflammation of granulomatosis with polyangiitis (Wegener's granulomatosis)

Jin Kyun Park, Frederic Askin

Research output: Contribution to journalArticlepeer-review

Abstract

Objective. To determine whether generation of osteoclast-like multinucleated giant cells (MNG) is a general feature of granulomatosis with polyangiitis (GPA). Methods. MNG phenotype of GPA sinus was examined by immunohistochemistry using antibodies against CD68, and cathepsin K. Tartrate resistant acid phosphatase (TRAP) expression was assessed by enzymatic colour reaction. Effects of bacterial wall components peptidoglycan (PGN) or lipoteichoic acid (LTA) on TRAP+ MNG formation were determined. Results. Tissue infiltrating MNGs in sinus expressed CD68, TRAP, and cathepsin K. They were strikingly less frequent in sinus than in lung lesions (23.1% vs. 70%, p=0.04). PGN and LTA inhibited MNG formation in a dose-dependent manner. Conclusions. While the generation of osteoclast-like MNGs is an intrinsic feature of GPA, MNGs are rare in sinonasal GPA lesions. Inhibition of MNG formation by bacterial cell wall components may occur preferentially in this sinonasal microenvironment, and contribute to these striking regional pathological differences.

Original languageEnglish (US)
Pages (from-to)S28-S31
JournalClinical and experimental rheumatology
Volume31
Issue numberSUPPL.75
StatePublished - Jun 5 2013

Keywords

  • Bacteria
  • Granulomatosis with polyangiitis (wegener's granulomatosis)
  • Multinucleated giant cells
  • Osteoclasts
  • Tartrate resistant acid phosphatase (TRAP)

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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