Orthogonal time-of-flight secondary ion mass spectrometric analysis of peptides using large gold clusters as primary ions

Agnès Tempez, J. A. Schultz, S. Della-Negra, J. Depauw, D. Jacquet, A. Novikov, Y. Lebeyec, M. Pautrat, M. Caroff, M. Ugarov, H. Bensaoula, M. Gonin, K. Fuhrer, Amina Woods

Research output: Contribution to journalArticle

Abstract

Secondary ion mass spectrometry (SIMS) for biomolecular analysis is greatly enhanced by the instrumental combination of orthogonal extraction time-of-flight mass spectrometry with massive gold cluster primary ion bombardment. Precursor peptide molecular ion yield enhancements of 1000, and signal-to-noise improvements of up to 20, were measured by comparing SIMS spectra obtained using Au+ and massive Au4004+ cluster primary ion bombardment of neat films of the neuropeptide fragment dynorphin 1-7. Remarkably low damage cross-sections were also measured from dynorphin 1-7 and gramicidin S during prolonged bombardment with 40 keV Au 4004+. For gramicidin S, the molecular ion yield increases slightly as a function of Au4004+ beam fluence up to at least 2 × 1013 Au4004+/cm 2. This is in marked contrast to the rapid decrease observed when bombarding with ions such as Au5+ and Au9 +. When gramicidin S is impinged with Au5+, the molecular ion yield decreases by a factor of 10 after a fluence of only 8 × 1012ions/cm2. Comparison of these damage cross-sections implies that minimal surface damage occurs during prolonged Au4004+ bombardment. Several practical analytical implications are drawn from these observations.

Original languageEnglish (US)
Pages (from-to)371-376
Number of pages6
JournalRapid communications in mass spectrometry : RCM
Volume18
Issue number4
StatePublished - 2004

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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    Tempez, A., Schultz, J. A., Della-Negra, S., Depauw, J., Jacquet, D., Novikov, A., Lebeyec, Y., Pautrat, M., Caroff, M., Ugarov, M., Bensaoula, H., Gonin, M., Fuhrer, K., & Woods, A. (2004). Orthogonal time-of-flight secondary ion mass spectrometric analysis of peptides using large gold clusters as primary ions. Rapid communications in mass spectrometry : RCM, 18(4), 371-376.