Orthogonal time-of-flight secondary ion mass spectrometric analysis of peptides using large gold clusters as primary ions

Agnès Tempez, J. A. Schultz, S. Della-Negra, J. Depauw, D. Jacquet, A. Novikov, Y. Lebeyec, M. Pautrat, M. Caroff, M. Ugarov, H. Bensaoula, M. Gonin, K. Fuhrer, Amina Woods

Research output: Contribution to journalArticle

Abstract

Secondary ion mass spectrometry (SIMS) for biomolecular analysis is greatly enhanced by the instrumental combination of orthogonal extraction time-of-flight mass spectrometry with massive gold cluster primary ion bombardment. Precursor peptide molecular ion yield enhancements of 1000, and signal-to-noise improvements of up to 20, were measured by comparing SIMS spectra obtained using Au+ and massive Au4004+ cluster primary ion bombardment of neat films of the neuropeptide fragment dynorphin 1-7. Remarkably low damage cross-sections were also measured from dynorphin 1-7 and gramicidin S during prolonged bombardment with 40 keV Au 4004+. For gramicidin S, the molecular ion yield increases slightly as a function of Au4004+ beam fluence up to at least 2 × 1013 Au4004+/cm 2. This is in marked contrast to the rapid decrease observed when bombarding with ions such as Au5+ and Au9 +. When gramicidin S is impinged with Au5+, the molecular ion yield decreases by a factor of 10 after a fluence of only 8 × 1012ions/cm2. Comparison of these damage cross-sections implies that minimal surface damage occurs during prolonged Au4004+ bombardment. Several practical analytical implications are drawn from these observations.

Original languageEnglish (US)
Pages (from-to)371-376
Number of pages6
JournalRapid communications in mass spectrometry : RCM
Volume18
Issue number4
StatePublished - 2004

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Gramicidin
Gold
Ions
Dynorphins
Peptides
Secondary ion mass spectrometry
Ion bombardment
Neuropeptides
Mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Tempez, A., Schultz, J. A., Della-Negra, S., Depauw, J., Jacquet, D., Novikov, A., ... Woods, A. (2004). Orthogonal time-of-flight secondary ion mass spectrometric analysis of peptides using large gold clusters as primary ions. Rapid communications in mass spectrometry : RCM, 18(4), 371-376.

Orthogonal time-of-flight secondary ion mass spectrometric analysis of peptides using large gold clusters as primary ions. / Tempez, Agnès; Schultz, J. A.; Della-Negra, S.; Depauw, J.; Jacquet, D.; Novikov, A.; Lebeyec, Y.; Pautrat, M.; Caroff, M.; Ugarov, M.; Bensaoula, H.; Gonin, M.; Fuhrer, K.; Woods, Amina.

In: Rapid communications in mass spectrometry : RCM, Vol. 18, No. 4, 2004, p. 371-376.

Research output: Contribution to journalArticle

Tempez, A, Schultz, JA, Della-Negra, S, Depauw, J, Jacquet, D, Novikov, A, Lebeyec, Y, Pautrat, M, Caroff, M, Ugarov, M, Bensaoula, H, Gonin, M, Fuhrer, K & Woods, A 2004, 'Orthogonal time-of-flight secondary ion mass spectrometric analysis of peptides using large gold clusters as primary ions', Rapid communications in mass spectrometry : RCM, vol. 18, no. 4, pp. 371-376.
Tempez, Agnès ; Schultz, J. A. ; Della-Negra, S. ; Depauw, J. ; Jacquet, D. ; Novikov, A. ; Lebeyec, Y. ; Pautrat, M. ; Caroff, M. ; Ugarov, M. ; Bensaoula, H. ; Gonin, M. ; Fuhrer, K. ; Woods, Amina. / Orthogonal time-of-flight secondary ion mass spectrometric analysis of peptides using large gold clusters as primary ions. In: Rapid communications in mass spectrometry : RCM. 2004 ; Vol. 18, No. 4. pp. 371-376.
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