@article{ea23d45f0a9142c7b5ed9f26629958c5,
title = "Origins and clonal convergence of gastrointestinal IgE+ B cells in human peanut allergy",
abstract = "B cells in human food allergy have been studied predominantly in the blood. Little is known about IgE+ B cells or plasma cells in tissues exposed to dietary antigens. We characterized IgE+ clones in blood, stomach, duodenum, and esophagus of 19 peanut-allergic patients, using high-throughput DNA sequencing. IgE+ cells in allergic patients are enriched in stomach and duodenum, and have a plasma cell phenotype. Clonally related IgE+ and non-IgE-expressing cell frequencies in tissues suggest local isotype switching, including transitions between IgA and IgE isotypes. Highly similar antibody sequences specific for peanut allergen Ara h 2 are shared between patients, indicating that common immunoglobulin genetic rearrangements may contribute to pathogenesis. These data define the gastrointestinal tract as a reservoir of IgE+ B lineage cells in food allergy.",
author = "Hoh, {Ramona A.} and Joshi, {Shilpa A.} and Lee, {Ji Yeun} and Martin, {Brock A.} and Sushama Varma and Shirley Kwok and Nielsen, {Sandra C.A.} and Parastu Nejad and Emily Haraguchi and Dixit, {Priya S.} and Shutthanandan, {Swetha V.} and Roskin, {Krishna M.} and Wenming Zhang and Dana Tupa and Bunning, {Bryan J.} and Monali Manohar and Robert Tibshirani and Fernandez-Becker, {Nielsen Q.} and Neeraja Kambham and West, {Robert B.} and Hamilton, {Robert G.} and Mindy Tsai and Galli, {Stephen J.} and Chinthrajah, {Rebecca S.} and Nadeau, {Kari C.} and Boyd, {Scott D.}",
note = "Funding Information: This study was supported by the NIH/National Institute of Allergy and Infectious Diseases (NIAID) (grants U19 AI104209 to S.J.G., K.C.N., S.D.B., R.S.C., and N.Q.F.-B.; grant R01 AI125567 to S.D.B. and S.J.G.; grants R01 AI127877 and R01 AI130398 to S.D.B.; grants R01 AI140134, R01 HL118612, U01 AI140498, and UM1 AI130839 to K.C.N.), a Big Data for Human Health Seed Grant from the Li Ka Shing Foundation (to S.D.B. and S.A.J.), an endowment from the Crown Family Foundation (to S.D.B.), the Sean N. Parker Center for Allergy and Asthma Research at Stanford University Medical Center (to K.C.N.), Food Allergy Research and Education Center of Excellence (to K.C.N.), the Myra Reinhard Foundation (to K.C.N.), End Allergies Together (to K.C.N.), the Hartman Family Foundation (to K.C.N.), the Naddisy Foundation (to K.C.N.), and the Levin Family Foundation (to K.C.N.). Publisher Copyright: Copyright {\textcopyright} 2020 The Authors, some rights reserved.",
year = "2020",
doi = "10.1126/sciimmunol.aay4209",
language = "English (US)",
volume = "5",
journal = "Science immunology",
issn = "2470-9468",
publisher = "American Association for the Advancement of Science",
number = "45",
}