Original article: Differentiation dependent expression of P-glycoprotein in the normal and neoplastic human kidney

C. K. Van Kalken, P. Der Van Valk, M. M N Hadisaputro, R. Pieters, H. J. Broxterman, C. M. Kuiper, G. L. Scheffer, A. J P Veerman, C. J L M Meyer, R. J. Scheper, H. M. Pinedo

Research output: Contribution to journalArticlepeer-review

Abstract

Summary: Adult renal cell carcinoma (RCC) is clinically resistant to chemotherapy. However, in nephroblastoma (NBL) chemotherapy has increased survival dramatically. We studied the P-glycoprotein (P-gp) expression of 18 RCC and 9 NBL as well as 1 benign renal adenoma and fetal renal tissue using three different monoclonal antibodies (MRK-16, C-219, JSB-1). P-gp was found positive with all three antibodies in 12/18 RCC, while only 2 tumors were completely negative. Staining varied with respect to intensity and number of positive cells [5%-90%]. Intense staining was seen at the apical side of malignant tubules in well differentiated parts of RCC and in tubular structures of the benign renal adenoma. Poorly differentiated parts of the tumors showed less staining. In NBL blastemal parts were negative. In 4/8 specimens showing focal epithelial differentiation, however, the luminal side of more differentiated tubular structures did stain, strongly resembling P-gp staining in the developing fetal human kidney. These results indicate that P-gp expression in normal (fetal) human kidney as well as in benign and malignant tumors derived from this organ depends on the degree of differentiation of tubules, which may have implications for chemotherapy sensitivity in both malignant tumors.

Original languageEnglish (US)
Pages (from-to)55-62
Number of pages8
JournalAnnals of Oncology
Volume2
Issue number1
StatePublished - Jan 1991
Externally publishedYes

Keywords

  • Differentiation
  • Fetal kidney
  • Immunohistochemistry
  • Nephroblastoma
  • P-glycoprotein
  • Renal cell cancer

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Hematology
  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Original article: Differentiation dependent expression of P-glycoprotein in the normal and neoplastic human kidney'. Together they form a unique fingerprint.

Cite this