Organization of the CTX prophage in environmental isolates of Vibrio mimicus

Mohammad Sirajul Islam; Mohammed Ziaur Rahman; Sirajul Islam Khan; Zahid Hayat Mahmud; Thandavarayan Ramamurthy; Gopinath Balakrish Nair; Richard Bradley Sack; David Allen Sack

doi:10.1111/j.1348-0421.2005.tb03668.x

Organization of the CTX prophage in environmental isolates of Vibrio mimicus

Mohammad Sirajul Islam, Mohammed Ziaur Rahman, Sirajul Islam Khan, Zahid Hayat Mahmud, Thandavarayan Ramamurthy, Gopinath Balakrish Nair, Richard Bradley Sack, David Allen Sack

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The organization of the CTX prophage in environmental strains of Vibrio mimicus was investigated. Sixteen hundred non-sucrose fermenting vibrios were examined for ctx gene by hybridization. Out of 1,600 isolates, 6 V. mimicus isolates contained ctxA gene. The organization of CTX prophage was determined by RFLP using ctxA probe. The CTX prophage integrated at a single site in V. mimicus genome which was present as a single copy flanked by at least a single RS element. Ribotype pattern revealed that a particular clone of V. mimicus acquired the CTXφ in the aquatic environment. This study demonstrated that V. mimicus could act as a reservoir of CTXφ in the aquatic environment.

Original language	English (US)
Pages (from-to)	779-784
Number of pages	6
Journal	Microbiology and Immunology
Volume	49
Issue number	8
DOIs	https://doi.org/10.1111/j.1348-0421.2005.tb03668.x
State	Published - 2005
Externally published	Yes

Keywords

Ctx gene
Environment
Genetic element
Vibrio mimicus

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.2005.tb03668.x

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title = "Organization of the CTX prophage in environmental isolates of Vibrio mimicus",

abstract = "The organization of the CTX prophage in environmental strains of Vibrio mimicus was investigated. Sixteen hundred non-sucrose fermenting vibrios were examined for ctx gene by hybridization. Out of 1,600 isolates, 6 V. mimicus isolates contained ctxA gene. The organization of CTX prophage was determined by RFLP using ctxA probe. The CTX prophage integrated at a single site in V. mimicus genome which was present as a single copy flanked by at least a single RS element. Ribotype pattern revealed that a particular clone of V. mimicus acquired the CTXφ in the aquatic environment. This study demonstrated that V. mimicus could act as a reservoir of CTXφ in the aquatic environment.",

keywords = "Ctx gene, Environment, Genetic element, Vibrio mimicus",

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doi = "10.1111/j.1348-0421.2005.tb03668.x",

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T1 - Organization of the CTX prophage in environmental isolates of Vibrio mimicus

AU - Islam, Mohammad Sirajul

AU - Rahman, Mohammed Ziaur

AU - Khan, Sirajul Islam

AU - Mahmud, Zahid Hayat

AU - Ramamurthy, Thandavarayan

AU - Nair, Gopinath Balakrish

AU - Sack, Richard Bradley

AU - Sack, David Allen

PY - 2005

Y1 - 2005

N2 - The organization of the CTX prophage in environmental strains of Vibrio mimicus was investigated. Sixteen hundred non-sucrose fermenting vibrios were examined for ctx gene by hybridization. Out of 1,600 isolates, 6 V. mimicus isolates contained ctxA gene. The organization of CTX prophage was determined by RFLP using ctxA probe. The CTX prophage integrated at a single site in V. mimicus genome which was present as a single copy flanked by at least a single RS element. Ribotype pattern revealed that a particular clone of V. mimicus acquired the CTXφ in the aquatic environment. This study demonstrated that V. mimicus could act as a reservoir of CTXφ in the aquatic environment.

AB - The organization of the CTX prophage in environmental strains of Vibrio mimicus was investigated. Sixteen hundred non-sucrose fermenting vibrios were examined for ctx gene by hybridization. Out of 1,600 isolates, 6 V. mimicus isolates contained ctxA gene. The organization of CTX prophage was determined by RFLP using ctxA probe. The CTX prophage integrated at a single site in V. mimicus genome which was present as a single copy flanked by at least a single RS element. Ribotype pattern revealed that a particular clone of V. mimicus acquired the CTXφ in the aquatic environment. This study demonstrated that V. mimicus could act as a reservoir of CTXφ in the aquatic environment.

KW - Ctx gene

KW - Environment

KW - Genetic element

KW - Vibrio mimicus

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JO - Microbiology and Immunology

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IS - 8

ER -

Organization of the CTX prophage in environmental isolates of Vibrio mimicus

Abstract

Keywords

ASJC Scopus subject areas

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