Organization and expression of the gene encoding chick kainate binding protein, a member of the glutamate receptor family

Paul Gregor, Xiaodong Yang, Itzchak Mano, Motohide Takemura, Vivian I. Teichberg, George R. Uhl

Research output: Contribution to journalArticle

Abstract

The gene encoding chick cerebellar Bergmann glia-specific kainate binding protein (chKBP), has been isolated, characterized and expressed in heterologous systems. The structural gene spans 11.2 kb and contains 11 exons and 10 introns. Several of the exons encode specific receptor domains, including each of the predicted transmembrane regions. Exon/intron boundaries flanking the second, putative channel-forming transmembrane domain are conserved between chKBP and other glutamate/kainate receptor subunits. The putative promoter region 5′ to the first exon displays high GC content and TATA, CAAT and API consensus sequences. Transcription of the chKBP gene is evident prior to full cerebellar cortical maturation. Transcripts are abundant in cells consistent with Bergmann glia, as revealed by in situ hybridization. Transfection of 293 kidney cell cultures with chKBP cDNA or chKBP gene expression constructs confers CNQX-sensitive kainate binding with the pharmacological specificity displayed by both chKBP and kainate receptors. However, expression of the same constructs in Xenopus oocytes fails to yield detectable agonist-activated currents.

Original languageEnglish (US)
Pages (from-to)179-186
Number of pages8
JournalMolecular Brain Research
Volume16
Issue number3-4
DOIs
StatePublished - 1992

Fingerprint

Kainic Acid
Glutamate Receptors
Exons
Carrier Proteins
Kainic Acid Receptors
Gene Expression
Neuroglia
Introns
Genes
6-Cyano-7-nitroquinoxaline-2,3-dione
Consensus Sequence
Base Composition
Xenopus
Genetic Promoter Regions
Oocytes
In Situ Hybridization
Transfection
Complementary DNA
Cell Culture Techniques
Pharmacology

Keywords

  • Bergmann glia
  • Cerebellum
  • Gene structure
  • Kainate receptor gene

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Organization and expression of the gene encoding chick kainate binding protein, a member of the glutamate receptor family. / Gregor, Paul; Yang, Xiaodong; Mano, Itzchak; Takemura, Motohide; Teichberg, Vivian I.; Uhl, George R.

In: Molecular Brain Research, Vol. 16, No. 3-4, 1992, p. 179-186.

Research output: Contribution to journalArticle

Gregor, Paul ; Yang, Xiaodong ; Mano, Itzchak ; Takemura, Motohide ; Teichberg, Vivian I. ; Uhl, George R. / Organization and expression of the gene encoding chick kainate binding protein, a member of the glutamate receptor family. In: Molecular Brain Research. 1992 ; Vol. 16, No. 3-4. pp. 179-186.
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