@article{950b0c429d9b41d59b30f9f59cca7f55,
title = "Organelle-specific, rapid induction of molecular activities and membrane tethering",
abstract = "Using new chemically inducible dimerization probes, we generated a system to rapidly target proteins to individual intracellular organelles. Using this system, we activated Ras GTPase at distinct intracellular locations and induced tethering of membranes from two organelles, endoplasmic reticulum and mitochondria. Innovative techniques to rapidly perturb molecular activities and organelle-organelle communications at precise locations and timing will provide powerful strategies to dissect spatiotemporally complex biological processes.",
author = "Toru Komatsu and Igor Kukelyansky and McCaffery, {J. Michael} and Tasuku Ueno and Varela, {Lidenys C.} and Takanari Inoue",
note = "Funding Information: We thank B. Wattenberg (University of Louisville) for Cb5/MoA plasmids; N. Ishihara (Toyko Medical and Dental University) for Tom20 plasmid; C. Machamer (Johns Hopkins University) for Giantin plasmid; W.D. Heo (Korea Advanced Institute of Science and Technology) for RasGRF plasmid; F. Tsuruta (University of Tsukuba) for LAMP plasmid; M. Fivaz (Duke-National University of Singapore Graduate Medical School Singapore) YFP-RBD plasmid; and T. Wandless (Stanford University) for providing rapamycin analogs (iRap, synthetic ligand of FKBP, FK506M); F. Fernandez, M. Fivaz, M. Meffert, J. Zhang, V. Sample, D. Montell, M. Caterina, P. Devreotes, A. Ewald and D. Robinson for critical review of the manuscript; R. Pagano for helpful comments on PS assays; and Z. Wei, W. Wong, K. Venkatachalam and J. Cordon for a technical assistance with western blot analyses. Supported in part by US National Institutes of Health (MH084691 to T.I., NCRR1S10RR023454-01 to J.M.M.). T.K. and T.U. are recipients of a fellowship from Japanese Society for the Promotion of Science.",
year = "2010",
month = mar,
doi = "10.1038/nmeth.1428",
language = "English (US)",
volume = "7",
pages = "206--208",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "3",
}