Organelle-specific, rapid induction of molecular activities and membrane tethering

Toru Komatsu, Igor Kukelyansky, J. Michael McCaffery, Tasuku Ueno, Lidenys C. Varela, Takanari Inoue

Research output: Contribution to journalArticlepeer-review


Using new chemically inducible dimerization probes, we generated a system to rapidly target proteins to individual intracellular organelles. Using this system, we activated Ras GTPase at distinct intracellular locations and induced tethering of membranes from two organelles, endoplasmic reticulum and mitochondria. Innovative techniques to rapidly perturb molecular activities and organelle-organelle communications at precise locations and timing will provide powerful strategies to dissect spatiotemporally complex biological processes.

Original languageEnglish (US)
Pages (from-to)206-208
Number of pages3
JournalNature Methods
Issue number3
StatePublished - Mar 2010

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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