Ordering the final events in yeast exocytosis

Eric Grote, Chavela M. Carr, Peter J. Novick

Research output: Contribution to journalArticle

Abstract

In yeast, assembly of exocytic soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor (SNARE) complexes between the secretory vesicle SNARE Sncp and the plasma membrane SNAREs Ssop and Sec9p occurs at a late stage of the exocytic reaction. Mutations that block either secretory vesicle delivery or tethering prevent SNARE complex assembly and the localization of Sec1p, a SNARE complex binding protein, to sites of secretion. By contrast, wild-type levels of SNARE complexes persist in the sec1-1 mutant after a secretory block is imposed, suggesting a role for Sec1p after SNARE complex assembly. In the sec18-1 mutant, cisoSNARE complexes containing surface-accessible Sncp accumulate in the plasma membrane. Thus, one function of Sec18p is to disassemble SNARE complexes on the postfusion membrane.

Original languageEnglish (US)
Pages (from-to)439-451
Number of pages13
JournalJournal of Cell Biology
Volume151
Issue number2
DOIs
StatePublished - Oct 16 2000
Externally publishedYes

Fingerprint

SNARE Proteins
Exocytosis
Yeasts
Secretory Vesicles
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Cell Membrane
Carrier Proteins
Mutation
Membranes

Keywords

  • Exocyst
  • Membrane fusion
  • NSF
  • Sec1
  • SNAREs

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ordering the final events in yeast exocytosis. / Grote, Eric; Carr, Chavela M.; Novick, Peter J.

In: Journal of Cell Biology, Vol. 151, No. 2, 16.10.2000, p. 439-451.

Research output: Contribution to journalArticle

Grote, E, Carr, CM & Novick, PJ 2000, 'Ordering the final events in yeast exocytosis', Journal of Cell Biology, vol. 151, no. 2, pp. 439-451. https://doi.org/10.1083/jcb.151.2.439
Grote, Eric ; Carr, Chavela M. ; Novick, Peter J. / Ordering the final events in yeast exocytosis. In: Journal of Cell Biology. 2000 ; Vol. 151, No. 2. pp. 439-451.
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