Oral fluid/plasma cannabinoid ratios following controlled oral THC and smoked cannabis administration

Dayong Lee, Ryan G Vandrey, Garry Milman, Mateus Bergamaschi, Damodara R. Mendu, Jeannie A. Murray, Allan J. Barnes, Marilyn A. Huestis

Research output: Contribution to journalArticle

Abstract

Oral fluid (OF) is a valuable biological alternative for clinical and forensic drug testing. Evaluating OF to plasma (OF/P) cannabinoid ratios provides important pharmacokinetic data on the disposition of drug and factors influencing partition between matrices. Eleven chronic cannabis smokers resided on a closed research unit for 51 days. There were four 5-day sessions of 0, 30, 60, and 120 mg oral â̂†9-tetrahydrocannabinol (THC)/day followed by a five-puff smoked cannabis challenge on Day 5. Each session was separated by 9 days ad libitum cannabis smoking. OF and plasma specimens were analyzed for THC and metabolites. During ad libitum smoking, OF/P THC ratios were high (median, 6.1; range, 0.2-348.5) within 1 h after last smoking, decreasing to 0.1-20.7 (median, 2.1) by 13.0-17.1 h. OF/P THC ratios also decreased during 5-days oral THC dosing, and after the smoked cannabis challenge, median OF/P THC ratios decreased from 1.4 to 5.5 (0.04-245.6) at 0.25 h to 0.12 to 0.17 (0.04-5.1) at 10.5 h post-smoking. In other studies, longer exposure to more potent cannabis smoke and oromucosal cannabis spray was associated with increased OF/P THC peak ratios. Median OF/P 11-nor-9-carboxy-THC (THCCOOH) ratios were 0.3-2.5 (range, 0.1-14.7) ng/μg, much more consistent in various dosing conditions over time. OF/P THC, but not THCCOOH, ratios were significantly influenced by oral cavity contamination after smoking or oromucosal spray of cannabinoid products, followed by time-dependent decreases. Establishing relationships between OF and plasma cannabinoid concentrations is essential for making inferences of impairment or other clinical outcomes from OF concentrations.

Original languageEnglish (US)
Pages (from-to)7269-7279
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume405
Issue number23
DOIs
StatePublished - Sep 2013

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Dronabinol
Cannabinoids
Cannabis
Plasmas
Fluids
Smoking
Marijuana Smoking
Smoke
Pharmaceutical Preparations
Pharmacokinetics
Mouth
Metabolites
Contamination

Keywords

  • Cannabis
  • Delta9-tetrahydrocannabinol
  • Marijuana
  • Oral fluid
  • Plasma
  • Ratio

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Oral fluid/plasma cannabinoid ratios following controlled oral THC and smoked cannabis administration. / Lee, Dayong; Vandrey, Ryan G; Milman, Garry; Bergamaschi, Mateus; Mendu, Damodara R.; Murray, Jeannie A.; Barnes, Allan J.; Huestis, Marilyn A.

In: Analytical and Bioanalytical Chemistry, Vol. 405, No. 23, 09.2013, p. 7269-7279.

Research output: Contribution to journalArticle

Lee, Dayong ; Vandrey, Ryan G ; Milman, Garry ; Bergamaschi, Mateus ; Mendu, Damodara R. ; Murray, Jeannie A. ; Barnes, Allan J. ; Huestis, Marilyn A. / Oral fluid/plasma cannabinoid ratios following controlled oral THC and smoked cannabis administration. In: Analytical and Bioanalytical Chemistry. 2013 ; Vol. 405, No. 23. pp. 7269-7279.
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