Oral administration of human serum immunoglobulin in immunodeficient patients with viral gastroenteritis. A pharmacokinetic and functional analysis

G. A. Losonsky, J. P. Johnson, J. A. Winkelstein, R. H. Yolken

Research output: Contribution to journalArticlepeer-review

Abstract

We examined the pharmacokinetics and immunological activity of human serum immunoglobulins (HSG) possessing anti-rotavirus activity which were orally administered to three children with primary immunodeficiency syndromes and prolonged gastrointestinal excretion of rotavirus. Detailed analysis of the excretion of immunoglobulins labeled with biotin or I125 revealed that ~50% of the recovered radioactivity was excreted in the stools over a 3-d period. Approximately half of the excreted radioactivity recovered in the stool was in a macromolecular form with immunological activity. The remainder of the recovered radioactivity was excreted in the urine as low molecular weight fragments of free iodide. In addition, immunological and chromatographic analyses revealed that the oral administration of HSG resulted in the generation of rotavirus-specific immune complexes in the gastrointestinal tract with a subsequent decrease in the presence of uncomplexed rotavirus antigen. These studies indicate that orally administered HSG can survive passage in the gastrointestinal tract in an immunologically active form, and that the oral administration of immunoglobulins with specific reactivities has potential for the prevention or treatment of gastrointestinal infections.

Original languageEnglish (US)
Pages (from-to)2362-2367
Number of pages6
JournalJournal of Clinical Investigation
Volume76
Issue number6
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Medicine(all)

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