Optimization of the production of hcgβ-like material by caski human cervical carcinoma cells in roller bottle culture

Robert O. Hussa, Roland A. Pattillo, Anna C.F. Ruckert, Martha L. Rinke, Joseph P. Cortesi, Henry G. Fein

Research output: Contribution to journalArticlepeer-review

Abstract

This study was aimed at optimizing large-scale roller bottle culture conditions for CaSki human cervical carcinoma cells, to produce ectopic hCGβ-like material in quantities sufficient for subsequent characterization studies. Several cell culture techniques contributed to the achievement of this goal; (1) use of serum-free culture medium; (2) use of intermittent recovery periods in presence of serum; and (3) ultrafiltration of the serum-free medium pool for initial concentration of 100-fold.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalOncology (Switzerland)
Volume43
Issue number1
DOIs
StatePublished - Jan 1 1986

Keywords

  • Cell culture
  • Cervical carcinoma
  • Ectopic hCGβ

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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