Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazid

Haifeng Zeng, Jiadi Xu, Joseph S Gillen, Michael T Mcmahon, Dmitri Artemov, Jean Max Tyburn, Joost A B Lohman, Ryan E. Mewis, Kevin D. Atkinson, Gary G R Green, Simon B. Duckett, Peter C Van Zijl

Research output: Contribution to journalArticle

Abstract

Hyperpolarization produces nuclear spin polarization that is several orders of magnitude larger than that achieved at thermal equilibrium thus providing extraordinary contrast and sensitivity. As a parahydrogen induced polarization (PHIP) technique that does not require chemical modification of the substrate to polarize, Signal Amplification by Reversible Exchange (SABRE) has attracted a lot of attention. Using a prototype parahydrogen polarizer, we polarize two drugs used in the treatment of tuberculosis, namely pyrazinamide and isoniazid. We examine this approach in four solvents, methanol-d4, methanol, ethanol and DMSO and optimize the polarization transfer magnetic field strength, the temperature as well as intensity and duration of hydrogen bubbling to achieve the best overall signal enhancement and hence hyperpolarization level.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalJournal of Magnetic Resonance
Volume237
DOIs
StatePublished - 2013

Fingerprint

tuberculosis
Pyrazinamide
Isoniazid
Amplification
Methanol
Tuberculosis
drugs
Polarization
Spin polarization
Contrast Sensitivity
optimization
Chemical modification
polarization
Magnetic Fields
methyl alcohol
Dimethyl Sulfoxide
Pharmaceutical Preparations
Hydrogen
Ethanol
Hot Temperature

Keywords

  • Hyperpolarization
  • Isoniazid
  • Mycobacterium Tuberculosis
  • Parahydrogen
  • Pyrazinamide
  • SABRE

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics

Cite this

Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazid. / Zeng, Haifeng; Xu, Jiadi; Gillen, Joseph S; Mcmahon, Michael T; Artemov, Dmitri; Tyburn, Jean Max; Lohman, Joost A B; Mewis, Ryan E.; Atkinson, Kevin D.; Green, Gary G R; Duckett, Simon B.; Van Zijl, Peter C.

In: Journal of Magnetic Resonance, Vol. 237, 2013, p. 73-78.

Research output: Contribution to journalArticle

Zeng, Haifeng ; Xu, Jiadi ; Gillen, Joseph S ; Mcmahon, Michael T ; Artemov, Dmitri ; Tyburn, Jean Max ; Lohman, Joost A B ; Mewis, Ryan E. ; Atkinson, Kevin D. ; Green, Gary G R ; Duckett, Simon B. ; Van Zijl, Peter C. / Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazid. In: Journal of Magnetic Resonance. 2013 ; Vol. 237. pp. 73-78.
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AU - Artemov, Dmitri

AU - Tyburn, Jean Max

AU - Lohman, Joost A B

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