Optimization of PEI/VEGF DNA polyplexes for potenital delivery from tissue engineering scaffolds

J. H. Van Zanten, Y. Har-El, J. Hanes

Research output: Contribution to journalConference article

Abstract

Non-viral vector formation by DNA complexation with cationic condensing agents is a self-assembly process driven primarily by electrostatic interactions and counterion release. DNA complexation kinetics influence three physical parameters that have a direct effect on gene delivery and expression efficiency: DNA complex geometric size, surface charge and density. In this study we demonstrate the utility of time resolved multiangle laser light scattering (TR-MALLS) for probing polyethylenimine (PEI) based polyplex formation kinetics with plasmid DNA. Our studies utilize plasmid DNA coding for VEGF, which may be used to enhance blood vessel in-growth into cell seeded polymeric scaffolds used in tissue engineering applications. We determined PEI/DNA complex size and density in real time and monitored vector stability in various liquid formulations. Parameters such as PEI molecular weight, N/P ratio and solution pH & ionic strength were investigated systematically. The ability to accurately measure polyplex size and density may lead to improvements in the design and control of non-viral gene delivery vectors and facilitate the determination of optimal formulations.

Original languageEnglish (US)
Pages (from-to)563-564
Number of pages2
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
StatePublished - Dec 1 2002
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Keywords

  • Gene therapy
  • Light scattering
  • Tissue engineering

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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