Optimization of fermentation conditions for the production of ethanol from sago starch using response surface methodology

B. V.V. Ratnam, M. Narasimha Rao, M. Damodar Rao, S. Subba Rao, C. Ayyanna

Research output: Contribution to journalArticlepeer-review

Abstract

The quantitative effects of temperature, pH and time of fermentation were investigated on simultaneous saccharification and fermentation (SSF) of ethanol from sago starch with glucoamylase (AMG) and Zymomonas mobilis ZM4 using a Box-Wilson central composite design protocol. The SSF process was studied using free enzyme and free cells and it was found that with sago starch, maximum ethanol concentration of 70.68 g/l was obtained using a starch concentration of 140 g/l, which represents an ethanol yield of 97.08%. The optimum conditions for the above yield were found to be a temperature of 36.74°C, pH of 5.02 and time of fermentation of 17 h. Thus by using the central composite design, it is possible to determine the accurate values of the fermentation parameters where maximum production of ethanol occurs.

Original languageEnglish (US)
Pages (from-to)523-526
Number of pages4
JournalWorld Journal of Microbiology and Biotechnology
Volume19
Issue number5
DOIs
StatePublished - Jul 1 2003

Keywords

  • Central composite design
  • Ethanol
  • Glucoamylase
  • Response surface methodology
  • Sago starch
  • Simultaneous saccharification and fermentation (SSF)
  • Zymomonas mobilis

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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