Optimization of automated matrix deposition for biomolecular mapping using a spotter

Alice M. Delvolve, Amina S. Woods

Research output: Contribution to journalArticlepeer-review

Abstract

Imaging mass spectrometry using matrix-assisted laser desorption/ionization allows the detailed mapping of biomolecules directly from tissue. Matrix deposition is the key step for successful imaging. The appropriate concentration and deposition of matrix is critical for extraction, desorption, and ionization of molecules from tissue without losing molecular localization. The main challenge to meet these criteria is to deposit matrix droplets homogeneously on the tissue section. This work shows how a chemical inkjet printer was used for this purpose resulting in the imaging of phosphatidylcholines and sulfatides. The intricacies involved in effective matrix deposition are discussed.

Original languageEnglish (US)
Pages (from-to)1046-1050
Number of pages5
JournalJournal of Mass Spectrometry
Volume46
Issue number10
DOIs
StatePublished - Oct 2011
Externally publishedYes

Keywords

  • chemical ink jet printer
  • imaging
  • lipids
  • mass spectrometry

ASJC Scopus subject areas

  • Spectroscopy

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