Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of this study were to quantify this effect and then to improve assay performance.
- Allele-specific PCR
- Low frequency variants
- Patient-specific HIV-1 consensus sequence
- Subtype consensus sequence
ASJC Scopus subject areas