Optimization and Stability Testing of Four Commercially Available Dried Blood Spot Devices for Estimating Measles and Rubella IgG Antibodies

Ojas Kaduskar, Vaishali Bhatt, Christine Prosperi, Kyla Hayford, Alvira Z. Hasan, Gururaj Rao Deshpande, Bipin Tilekar, Jeromie Wesley Vivian Thangaraj, Muthusamy Santhosh Kumar, Nivedita Gupta, Manoj V. Murhekar, William J. Moss, Sanjay M. Mehendale, Lucky Sangal, Gajanan Sapkala

Research output: Contribution to journalArticlepeer-review

Abstract

Blood collection using dried blood spots (DBS) provides an easier alternative to venipuncture for sample collection, transport, and storage but requires additional processing that can cause variability in results. Whole-blood samples spotted on four DBS devices and respective paired serum samples were tested for antimeasles and antirubella IgG antibody concentrations by enzyme immunoassay. Elution protocols for DBS devices were optimized for comparability relative to serum samples using 12 adult volunteers. Stability of DBS collected on HemaSpot HF was assessed under various temperature conditions (14, 22 to 25, and 45°C) at six time points (0, 7, 15, 30, 60, and 90 days) in a controlled laboratory setting using six adult volunteers. Devices were shipped and stored for 30 days at four settings with variable temperature and humidity conditions to assess the impact on antibody concentrations. Three DBS devices demonstrated comparable antibody concentrations with paired sera following optimization. Antibodies recovered from DBS were stable for at least 90 days at 4°C and for 30 days at ambient temperature (22 to 25°C) using the HemaSpot HF device. A drastic decline in antibody concentrations was observed at 45°C, resulting in quantitative and qualitative discrepancies by day 7. HemaSpot HF devices shipped to field sites and stored at ambient temperature and humidity resulted in quantitative, but not qualitative, variability. Measurement of antimeasles and antirubella IgG antibodies with DBS devices is an accurate alternative to testing serum, provided elution protocols are optimized. Stability of HemaSpot HF devices at ambient temperature enables broader use in surveys when serum processing and cold storage are not feasible. IMPORTANCE Dried blood spot (DBS) collection offers various advantages over conventional methods of blood collection, especially when collecting and transporting laboratory that can add to variability in results. We optimized a protocol to elute IgG antibodies against measles and rubella viruses in four DBS devices, demonstrating high concordance with paired venous sera for most devices. Extensive stability studies with various temperature and storage conditions in the laboratory and in the field were conducted using HemaSpot HF DBS devices prior to its use in one of the largest community-based measles and rubella serological surveys in the world

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalmSphere
Volume6
Issue number4
DOIs
StatePublished - Aug 2021

Keywords

  • ELISA
  • HemaSpot
  • dried blood spot
  • elution buffer
  • elution protocol
  • laboratory and field
  • measles
  • optimization
  • quantitative IgG titers
  • rubella
  • stability
  • temperature and humidity

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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