Optimal growth temperature for the isolation of Plesiomonas shigelloides, using various selective and differential agars

A. Huq; A. Akhtar; M. A.R. Chowdhury; D. A. Sack

doi:10.1139/m91-138

Optimal growth temperature for the isolation of Plesiomonas shigelloides, using various selective and differential agars

A. Huq, A. Akhtar, M. A.R. Chowdhury, D. A. Sack

Research output: Contribution to journal › Article › peer-review

Abstract

The growth characteristics of known strains of Plesiomonas shigelloides were compared with those of Aeromonas species (the major competing species in environmental waters) on plesiomonas differential agar, inositol brilliant green bile salt, and modified salmonella-shigella agar at incubation temperatures of 37, 42, and 44°C. Using local isolates from clinical and environmental sources, optimal growth conditions, as determined by colony counts and the colony characteristics, plesiomonas differential agar proved to be ideal when incubated at 44°C. Contrary to earlier recommendations for 48 h incubation, the colonies could be recognized readily after an incubation of 24 h.

Original language	English (US)
Pages (from-to)	800-802
Number of pages	3
Journal	Canadian Journal of Microbiology
Volume	37
Issue number	10
DOIs	https://doi.org/10.1139/m91-138
State	Published - Jan 1 1991
Externally published	Yes

Keywords

Plesiomonas
growth media
growth temperature

ASJC Scopus subject areas

Microbiology
Immunology
Applied Microbiology and Biotechnology
Molecular Biology
Genetics

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10.1139/m91-138

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title = "Optimal growth temperature for the isolation of Plesiomonas shigelloides, using various selective and differential agars",

abstract = "The growth characteristics of known strains of Plesiomonas shigelloides were compared with those of Aeromonas species (the major competing species in environmental waters) on plesiomonas differential agar, inositol brilliant green bile salt, and modified salmonella-shigella agar at incubation temperatures of 37, 42, and 44°C. Using local isolates from clinical and environmental sources, optimal growth conditions, as determined by colony counts and the colony characteristics, plesiomonas differential agar proved to be ideal when incubated at 44°C. Contrary to earlier recommendations for 48 h incubation, the colonies could be recognized readily after an incubation of 24 h.",

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AU - Chowdhury, M. A.R.

AU - Sack, D. A.

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AB - The growth characteristics of known strains of Plesiomonas shigelloides were compared with those of Aeromonas species (the major competing species in environmental waters) on plesiomonas differential agar, inositol brilliant green bile salt, and modified salmonella-shigella agar at incubation temperatures of 37, 42, and 44°C. Using local isolates from clinical and environmental sources, optimal growth conditions, as determined by colony counts and the colony characteristics, plesiomonas differential agar proved to be ideal when incubated at 44°C. Contrary to earlier recommendations for 48 h incubation, the colonies could be recognized readily after an incubation of 24 h.

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