Optical scatter imaging: Subcellular morphometry in situ with Fourier filtering

Nada N. Boustany, Scot C. Kuo, Nitish V. Thakor

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate a quantitative optical scatter imaging (OSI) technique, based on Fourier filtering, for detecting alterations in the size of particles with wavelength-scale dimensions. We generate our scatter image by taking the ratio of images collected at high and low numerical aperture in central dark-field microscopy. Such an image spatially encodes the ratio of wide to narrow angle scatter and hence provides a measure of local particle size. We validated OSI on sphere suspensions and live cells. In live cells, OSI revealed biochemically induced morphological changes that were not apparent in unprocessed differential interference contrast images. Unlike high-resolution imaging methods, OSI can provide size information for particles smaller than the camera's spatial resolution.

Original languageEnglish (US)
Pages (from-to)1063-1065
Number of pages3
JournalOptics Letters
Volume26
Issue number14
DOIs
StatePublished - Jul 15 2001

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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