Optical imaging of green fluorescent protein markers for tracking vascular gene expression: A feasibility study in human tissue-like phantoms

Ananda Kumar, Hunter H. Chen, Erin Long, Danming Wang, Xiaoming Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Vascular gene therapy is an exciting approach to the treatment of cardiovascular diseases. However, to date, there are no imaging modalities available for non-invasive detection of vascular gene expression. We have developed an optical imaging method to track vascular gene expression by detecting fluorescent signals emitted from arterial walls following gene transfer. To investigate the feasibility of this new technique, we performed experiments on a set of human tissue-like phantoms using a common biological marker in gene therapy, the green fluorescent protein (GFP). The phantoms were constructed to mimic the arterial geometry beneath a tissue layer. Human smooth muscle cells transfected with GFP were embedded in a capillary tube in the phantom. Monte Carlo modeling of the phantom experiment was performed to optimize the performance of the optical imaging system. We compared the fluence rates among three types of light beams, including ring beam, Gaussian beam, and flat beam. The results showed that our optical imaging system was able to detect fluorescent signals up to 5-mm depth in the phantom, and that flat beam geometry would produce the optimum fluorescence remittance. This study provides valuable insights for improvements to the optical imaging system and refinement of the new technique to non-invasively detect/track vascular gene expression.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsM.D. Kessler, G.J. Muller
Pages339-345
Number of pages7
Volume4623
DOIs
StatePublished - 2002
EventFunctional Monitoring and Drug-Tissue Interaction - San Jose, Ca, United States
Duration: Jan 21 2002Jan 24 2002

Other

OtherFunctional Monitoring and Drug-Tissue Interaction
CountryUnited States
CitySan Jose, Ca
Period1/21/021/24/02

Fingerprint

gene expression
Gene expression
Imaging systems
markers
Gene therapy
Tissue
gene therapy
proteins
Proteins
Imaging techniques
Gene transfer
Capillary tubes
Gaussian beams
Geometry
smooth muscle
muscle cells
capillary tubes
Muscle
biomarkers
geometry

Keywords

  • Gene therapy
  • Green fluorescent protein (GFP)
  • Monte Carlo simulation
  • Optical imaging
  • Photometry
  • Scattering
  • Vascular gene expression

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Kumar, A., Chen, H. H., Long, E., Wang, D., & Yang, X. (2002). Optical imaging of green fluorescent protein markers for tracking vascular gene expression: A feasibility study in human tissue-like phantoms. In M. D. Kessler, & G. J. Muller (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4623, pp. 339-345) https://doi.org/10.1117/12.469465

Optical imaging of green fluorescent protein markers for tracking vascular gene expression : A feasibility study in human tissue-like phantoms. / Kumar, Ananda; Chen, Hunter H.; Long, Erin; Wang, Danming; Yang, Xiaoming.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / M.D. Kessler; G.J. Muller. Vol. 4623 2002. p. 339-345.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kumar, A, Chen, HH, Long, E, Wang, D & Yang, X 2002, Optical imaging of green fluorescent protein markers for tracking vascular gene expression: A feasibility study in human tissue-like phantoms. in MD Kessler & GJ Muller (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4623, pp. 339-345, Functional Monitoring and Drug-Tissue Interaction, San Jose, Ca, United States, 1/21/02. https://doi.org/10.1117/12.469465
Kumar A, Chen HH, Long E, Wang D, Yang X. Optical imaging of green fluorescent protein markers for tracking vascular gene expression: A feasibility study in human tissue-like phantoms. In Kessler MD, Muller GJ, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4623. 2002. p. 339-345 https://doi.org/10.1117/12.469465
Kumar, Ananda ; Chen, Hunter H. ; Long, Erin ; Wang, Danming ; Yang, Xiaoming. / Optical imaging of green fluorescent protein markers for tracking vascular gene expression : A feasibility study in human tissue-like phantoms. Proceedings of SPIE - The International Society for Optical Engineering. editor / M.D. Kessler ; G.J. Muller. Vol. 4623 2002. pp. 339-345
@inproceedings{02c44a1d2e29465fa6093ca18b567bf2,
title = "Optical imaging of green fluorescent protein markers for tracking vascular gene expression: A feasibility study in human tissue-like phantoms",
abstract = "Vascular gene therapy is an exciting approach to the treatment of cardiovascular diseases. However, to date, there are no imaging modalities available for non-invasive detection of vascular gene expression. We have developed an optical imaging method to track vascular gene expression by detecting fluorescent signals emitted from arterial walls following gene transfer. To investigate the feasibility of this new technique, we performed experiments on a set of human tissue-like phantoms using a common biological marker in gene therapy, the green fluorescent protein (GFP). The phantoms were constructed to mimic the arterial geometry beneath a tissue layer. Human smooth muscle cells transfected with GFP were embedded in a capillary tube in the phantom. Monte Carlo modeling of the phantom experiment was performed to optimize the performance of the optical imaging system. We compared the fluence rates among three types of light beams, including ring beam, Gaussian beam, and flat beam. The results showed that our optical imaging system was able to detect fluorescent signals up to 5-mm depth in the phantom, and that flat beam geometry would produce the optimum fluorescence remittance. This study provides valuable insights for improvements to the optical imaging system and refinement of the new technique to non-invasively detect/track vascular gene expression.",
keywords = "Gene therapy, Green fluorescent protein (GFP), Monte Carlo simulation, Optical imaging, Photometry, Scattering, Vascular gene expression",
author = "Ananda Kumar and Chen, {Hunter H.} and Erin Long and Danming Wang and Xiaoming Yang",
year = "2002",
doi = "10.1117/12.469465",
language = "English (US)",
volume = "4623",
pages = "339--345",
editor = "M.D. Kessler and G.J. Muller",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Optical imaging of green fluorescent protein markers for tracking vascular gene expression

T2 - A feasibility study in human tissue-like phantoms

AU - Kumar, Ananda

AU - Chen, Hunter H.

AU - Long, Erin

AU - Wang, Danming

AU - Yang, Xiaoming

PY - 2002

Y1 - 2002

N2 - Vascular gene therapy is an exciting approach to the treatment of cardiovascular diseases. However, to date, there are no imaging modalities available for non-invasive detection of vascular gene expression. We have developed an optical imaging method to track vascular gene expression by detecting fluorescent signals emitted from arterial walls following gene transfer. To investigate the feasibility of this new technique, we performed experiments on a set of human tissue-like phantoms using a common biological marker in gene therapy, the green fluorescent protein (GFP). The phantoms were constructed to mimic the arterial geometry beneath a tissue layer. Human smooth muscle cells transfected with GFP were embedded in a capillary tube in the phantom. Monte Carlo modeling of the phantom experiment was performed to optimize the performance of the optical imaging system. We compared the fluence rates among three types of light beams, including ring beam, Gaussian beam, and flat beam. The results showed that our optical imaging system was able to detect fluorescent signals up to 5-mm depth in the phantom, and that flat beam geometry would produce the optimum fluorescence remittance. This study provides valuable insights for improvements to the optical imaging system and refinement of the new technique to non-invasively detect/track vascular gene expression.

AB - Vascular gene therapy is an exciting approach to the treatment of cardiovascular diseases. However, to date, there are no imaging modalities available for non-invasive detection of vascular gene expression. We have developed an optical imaging method to track vascular gene expression by detecting fluorescent signals emitted from arterial walls following gene transfer. To investigate the feasibility of this new technique, we performed experiments on a set of human tissue-like phantoms using a common biological marker in gene therapy, the green fluorescent protein (GFP). The phantoms were constructed to mimic the arterial geometry beneath a tissue layer. Human smooth muscle cells transfected with GFP were embedded in a capillary tube in the phantom. Monte Carlo modeling of the phantom experiment was performed to optimize the performance of the optical imaging system. We compared the fluence rates among three types of light beams, including ring beam, Gaussian beam, and flat beam. The results showed that our optical imaging system was able to detect fluorescent signals up to 5-mm depth in the phantom, and that flat beam geometry would produce the optimum fluorescence remittance. This study provides valuable insights for improvements to the optical imaging system and refinement of the new technique to non-invasively detect/track vascular gene expression.

KW - Gene therapy

KW - Green fluorescent protein (GFP)

KW - Monte Carlo simulation

KW - Optical imaging

KW - Photometry

KW - Scattering

KW - Vascular gene expression

UR - http://www.scopus.com/inward/record.url?scp=0036400198&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036400198&partnerID=8YFLogxK

U2 - 10.1117/12.469465

DO - 10.1117/12.469465

M3 - Conference contribution

AN - SCOPUS:0036400198

VL - 4623

SP - 339

EP - 345

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Kessler, M.D.

A2 - Muller, G.J.

ER -