Optical imaging of arrhythmias in tissue culture

Leslie Tung, Yibing Zhang

Research output: Contribution to journalArticle

Abstract

Background: Cardiac cell cultures are becoming an important experimental system of minimal complexity that captures many of the salient features of myocardial tissue function and are simple enough that tissue parameters can be controlled systematically. Fundamental mechanisms that underlie normal and pathological electrophysiology at the tissue level can be studied. Of particular interest are spiral waves, which underlie many tachyarrhythmias and fibrillation. Methods: Methods of patterned growth were used to control tissue structure, and contact fluorescence imaging was used to visualize the spread of electrical waves in confluent monolayers of neonatal rat ventricular cells stained with voltage-sensitive dye. Results: Work is summarized regarding anisotropy, multiarmed spirals, cocultures of cardiac cells and skeletal myoblasts or mesenchymal stem cells, mechanical excitation, attachment of spiral waves to small anatomical obstacles, perturbation of spiral waves by external electric fields, and structure-based facilitation of spiral wave formation. Conclusions: The cultured cell monolayer is a contemporary experimental model encompassing great versatility for basic studies of wavefront propagation and cardiac arrhythmias.

Original languageEnglish (US)
JournalJournal of Electrocardiology
Volume39
Issue number4 SUPPL.
DOIs
StatePublished - Oct 2006

Fingerprint

Optical Imaging
Cardiac Arrhythmias
Cardiac Myoblasts
Skeletal Myoblasts
Electrophysiology
Anisotropy
Coculture Techniques
Mesenchymal Stromal Cells
Tachycardia
Cultured Cells
Theoretical Models
Coloring Agents
Cell Culture Techniques
Growth

Keywords

  • Arrhythmia
  • Electrophysiology
  • Optical mapping
  • Reentry
  • Voltage-sensitive dye

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Optical imaging of arrhythmias in tissue culture. / Tung, Leslie; Zhang, Yibing.

In: Journal of Electrocardiology, Vol. 39, No. 4 SUPPL., 10.2006.

Research output: Contribution to journalArticle

@article{ffb0659b04dc488088fe273829a1579b,
title = "Optical imaging of arrhythmias in tissue culture",
abstract = "Background: Cardiac cell cultures are becoming an important experimental system of minimal complexity that captures many of the salient features of myocardial tissue function and are simple enough that tissue parameters can be controlled systematically. Fundamental mechanisms that underlie normal and pathological electrophysiology at the tissue level can be studied. Of particular interest are spiral waves, which underlie many tachyarrhythmias and fibrillation. Methods: Methods of patterned growth were used to control tissue structure, and contact fluorescence imaging was used to visualize the spread of electrical waves in confluent monolayers of neonatal rat ventricular cells stained with voltage-sensitive dye. Results: Work is summarized regarding anisotropy, multiarmed spirals, cocultures of cardiac cells and skeletal myoblasts or mesenchymal stem cells, mechanical excitation, attachment of spiral waves to small anatomical obstacles, perturbation of spiral waves by external electric fields, and structure-based facilitation of spiral wave formation. Conclusions: The cultured cell monolayer is a contemporary experimental model encompassing great versatility for basic studies of wavefront propagation and cardiac arrhythmias.",
keywords = "Arrhythmia, Electrophysiology, Optical mapping, Reentry, Voltage-sensitive dye",
author = "Leslie Tung and Yibing Zhang",
year = "2006",
month = "10",
doi = "10.1016/j.jelectrocard.2006.04.010",
language = "English (US)",
volume = "39",
journal = "Journal of Electrocardiology",
issn = "0022-0736",
publisher = "Churchill Livingstone",
number = "4 SUPPL.",

}

TY - JOUR

T1 - Optical imaging of arrhythmias in tissue culture

AU - Tung, Leslie

AU - Zhang, Yibing

PY - 2006/10

Y1 - 2006/10

N2 - Background: Cardiac cell cultures are becoming an important experimental system of minimal complexity that captures many of the salient features of myocardial tissue function and are simple enough that tissue parameters can be controlled systematically. Fundamental mechanisms that underlie normal and pathological electrophysiology at the tissue level can be studied. Of particular interest are spiral waves, which underlie many tachyarrhythmias and fibrillation. Methods: Methods of patterned growth were used to control tissue structure, and contact fluorescence imaging was used to visualize the spread of electrical waves in confluent monolayers of neonatal rat ventricular cells stained with voltage-sensitive dye. Results: Work is summarized regarding anisotropy, multiarmed spirals, cocultures of cardiac cells and skeletal myoblasts or mesenchymal stem cells, mechanical excitation, attachment of spiral waves to small anatomical obstacles, perturbation of spiral waves by external electric fields, and structure-based facilitation of spiral wave formation. Conclusions: The cultured cell monolayer is a contemporary experimental model encompassing great versatility for basic studies of wavefront propagation and cardiac arrhythmias.

AB - Background: Cardiac cell cultures are becoming an important experimental system of minimal complexity that captures many of the salient features of myocardial tissue function and are simple enough that tissue parameters can be controlled systematically. Fundamental mechanisms that underlie normal and pathological electrophysiology at the tissue level can be studied. Of particular interest are spiral waves, which underlie many tachyarrhythmias and fibrillation. Methods: Methods of patterned growth were used to control tissue structure, and contact fluorescence imaging was used to visualize the spread of electrical waves in confluent monolayers of neonatal rat ventricular cells stained with voltage-sensitive dye. Results: Work is summarized regarding anisotropy, multiarmed spirals, cocultures of cardiac cells and skeletal myoblasts or mesenchymal stem cells, mechanical excitation, attachment of spiral waves to small anatomical obstacles, perturbation of spiral waves by external electric fields, and structure-based facilitation of spiral wave formation. Conclusions: The cultured cell monolayer is a contemporary experimental model encompassing great versatility for basic studies of wavefront propagation and cardiac arrhythmias.

KW - Arrhythmia

KW - Electrophysiology

KW - Optical mapping

KW - Reentry

KW - Voltage-sensitive dye

UR - http://www.scopus.com/inward/record.url?scp=33749078309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749078309&partnerID=8YFLogxK

U2 - 10.1016/j.jelectrocard.2006.04.010

DO - 10.1016/j.jelectrocard.2006.04.010

M3 - Article

C2 - 17015066

AN - SCOPUS:33749078309

VL - 39

JO - Journal of Electrocardiology

JF - Journal of Electrocardiology

SN - 0022-0736

IS - 4 SUPPL.

ER -