TY - JOUR
T1 - Optical and force nanoscopy in microbiology
AU - Xiao, Jie
AU - Dufrêne, Yves F.
N1 - Funding Information:
Work in the Dufrêne team was supported by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement no. 693630), the National Fund for Scientific Research (FNRS), the FNRS-WELBIO (grant no. WELBIO-CR-2015A-05), the Federal Office for Scientific, Technical and Cultural Affairs (Interuniversity Poles of Attraction Programme), and the Research Department of the Communauté française de Belgique (Concerted research action). Y.F.D. is Research Director at the FNRS. Work in the Xiao lab is supported by National Institute of Health General Medicines 1R01GM086447-06, 1R01GM112008-01 (Multi-PI), National Science Foundation grant EAGER MCB1019000.
Publisher Copyright:
© 2016 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.
PY - 2016/10/26
Y1 - 2016/10/26
N2 - Microbial cells have developed sophisticated multicomponent structures and machineries to govern basic cellular processes, such as chromosome segregation, gene expression, cell division, mechanosensing, cell adhesion and biofilm formation. Because of the small cell sizes, subcellular structures have long been difficult to visualize using diffraction-limited light microscopy. During the last three decades, optical and force nanoscopy techniques have been developed to probe intracellular and extracellular structures with unprecedented resolutions, enabling researchers to study their organization, dynamics and interactions in individual cells, at the single-molecule level, from the inside out, and all the way up to cell-cell interactions in microbial communities. In this Review, we discuss the principles, advantages and limitations of the main optical and force nanoscopy techniques available in microbiology, and we highlight some outstanding questions that these new tools may help to answer.
AB - Microbial cells have developed sophisticated multicomponent structures and machineries to govern basic cellular processes, such as chromosome segregation, gene expression, cell division, mechanosensing, cell adhesion and biofilm formation. Because of the small cell sizes, subcellular structures have long been difficult to visualize using diffraction-limited light microscopy. During the last three decades, optical and force nanoscopy techniques have been developed to probe intracellular and extracellular structures with unprecedented resolutions, enabling researchers to study their organization, dynamics and interactions in individual cells, at the single-molecule level, from the inside out, and all the way up to cell-cell interactions in microbial communities. In this Review, we discuss the principles, advantages and limitations of the main optical and force nanoscopy techniques available in microbiology, and we highlight some outstanding questions that these new tools may help to answer.
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U2 - 10.1038/nmicrobiol.2016.186
DO - 10.1038/nmicrobiol.2016.186
M3 - Review article
C2 - 27782138
AN - SCOPUS:84992646538
VL - 1
JO - Nature Microbiology
JF - Nature Microbiology
SN - 2058-5276
IS - 11
M1 - 16186
ER -