Abstract
Microbial cells have developed sophisticated multicomponent structures and machineries to govern basic cellular processes, such as chromosome segregation, gene expression, cell division, mechanosensing, cell adhesion and biofilm formation. Because of the small cell sizes, subcellular structures have long been difficult to visualize using diffraction-limited light microscopy. During the last three decades, optical and force nanoscopy techniques have been developed to probe intracellular and extracellular structures with unprecedented resolutions, enabling researchers to study their organization, dynamics and interactions in individual cells, at the single-molecule level, from the inside out, and all the way up to cell-cell interactions in microbial communities. In this Review, we discuss the principles, advantages and limitations of the main optical and force nanoscopy techniques available in microbiology, and we highlight some outstanding questions that these new tools may help to answer.
| Original language | English (US) |
|---|---|
| Article number | 16186 |
| Journal | Nature Microbiology |
| Volume | 1 |
| Issue number | 11 |
| DOIs | |
| State | Published - Oct 26 2016 |
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ASJC Scopus subject areas
- Microbiology
- Applied Microbiology and Biotechnology
- Immunology
- Microbiology (medical)
- Cell Biology
- Genetics
Cite this
Optical and force nanoscopy in microbiology. / Xiao, Jie; Dufrêne, Yves F.
In: Nature Microbiology, Vol. 1, No. 11, 16186, 26.10.2016.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Optical and force nanoscopy in microbiology
AU - Xiao, Jie
AU - Dufrêne, Yves F.
PY - 2016/10/26
Y1 - 2016/10/26
N2 - Microbial cells have developed sophisticated multicomponent structures and machineries to govern basic cellular processes, such as chromosome segregation, gene expression, cell division, mechanosensing, cell adhesion and biofilm formation. Because of the small cell sizes, subcellular structures have long been difficult to visualize using diffraction-limited light microscopy. During the last three decades, optical and force nanoscopy techniques have been developed to probe intracellular and extracellular structures with unprecedented resolutions, enabling researchers to study their organization, dynamics and interactions in individual cells, at the single-molecule level, from the inside out, and all the way up to cell-cell interactions in microbial communities. In this Review, we discuss the principles, advantages and limitations of the main optical and force nanoscopy techniques available in microbiology, and we highlight some outstanding questions that these new tools may help to answer.
AB - Microbial cells have developed sophisticated multicomponent structures and machineries to govern basic cellular processes, such as chromosome segregation, gene expression, cell division, mechanosensing, cell adhesion and biofilm formation. Because of the small cell sizes, subcellular structures have long been difficult to visualize using diffraction-limited light microscopy. During the last three decades, optical and force nanoscopy techniques have been developed to probe intracellular and extracellular structures with unprecedented resolutions, enabling researchers to study their organization, dynamics and interactions in individual cells, at the single-molecule level, from the inside out, and all the way up to cell-cell interactions in microbial communities. In this Review, we discuss the principles, advantages and limitations of the main optical and force nanoscopy techniques available in microbiology, and we highlight some outstanding questions that these new tools may help to answer.
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U2 - 10.1038/nmicrobiol.2016.186
DO - 10.1038/nmicrobiol.2016.186
M3 - Review article
VL - 1
JO - Nature Microbiology
JF - Nature Microbiology
SN - 2058-5276
IS - 11
M1 - 16186
ER -