Opposite Responses of Rabbit and Human Globin mRNAs to Translational Inhibition by Cap Analogues

Susan H. Shakin, Stephen A. Liebhaber

Research output: Contribution to journalArticlepeer-review

Abstract

The translational efficiency of an mRNA may be determined at the step of translational initiation by the efficiency of its interaction with the cap binding protein complex. To further investigate the role of these interactions in translational control, we compare in vitro the relative sensitivities of rabbit and human α- and β-globin mRNAs to translational inhibition by cap analogues. We find that rabbit β-globin mRNA is more resistant to translational inhibition by cap analogues than rabbit α-globin mRNA, while in contrast, human β-globin mRNA is more sensitive to cap analogue inhibition than human a-globin mRNA. This opposite pattern of translational inhibition by cap analogues of the rabbit and human α- and β-globin mRNAs is unexpected as direct in vivo and in vitro comparisons of polysome profiles reveal parallel translational handling of the α- and β-globin mRNAs from these two species. This discordance between the relative translational sensitivities of these mRNAs to cap analogues and their relative ribosome loading activities suggests that cap-dependent events may not be rate limiting in steady-state globin translation.

Original languageEnglish (US)
Pages (from-to)7188-7193
Number of pages6
JournalBiochemistry
Volume26
Issue number22
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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