Opportunities and challenges of proteomics in pediatric patients: Circulating biomarkers after hematopoietic stem cell transplantation as a successful example

Sophie Paczesny, Christine Duncan, David Jacobsohn, Robert Krance, Kathryn Leung, Paul Carpenter, Catherine Bollard, Jamie Renbarger, Kenneth Cooke

Research output: Contribution to journalReview article

Abstract

Biomarkers have the potential to improve diagnosis and prognosis, facilitate-targeted treatment, and reduce health care costs. Thus, there is great hope that biomarkers will be integrated in all clinical decisions in the near future. A decade ago, the biomarker field was launched with great enthusiasm because MS revealed that blood contains a rich library of candidate biomarkers. However, biomarker research has not yet delivered on its promise due to several limitations: (i) improper sample handling and tracking as well as limited sample availability in the pediatric population, (ii) omission of appropriate controls in original study designs, (iii) lability and low abundance of interesting biomarkers in blood, and (iv) the inability to mechanistically tie biomarker presence to disease biology. These limitations as well as successful strategies to overcome them are discussed in this review. Several advances in biomarker discovery and validation have been made in hematopoietic stem cell transplantation, the current most effective tumor immunotherapy, and these could serve as examples for other conditions. This review provides fresh optimism that biomarkers clinically relevant in pediatrics are closer to being realized based on: (i) a uniform protocol for low-volume blood collection and preservation, (ii) inclusion of well-controlled independent cohorts, (iii) novel technologies and instrumentation with low analytical sensitivity, and (iv) integrated animal models for exploring potential biomarkers and targeted therapies.

Original languageEnglish (US)
Pages (from-to)837-850
Number of pages14
JournalProteomics - Clinical Applications
Volume8
Issue number11-12
DOIs
StatePublished - Dec 1 2014

Keywords

  • Biomarkers
  • Graft-versus-host disease
  • Hematopoietic stem cell transplantation
  • Pediatrics
  • Risk stratification

ASJC Scopus subject areas

  • Clinical Biochemistry

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