Open reading frame vpr of simian immunodeficiency virus encodes a virion-associated protein

Xiao Fang Yu, Michiko Matsuda, Max Essex, Tun Hou Lee

Research output: Contribution to journalArticle

Abstract

The genomes of simian immunodeficiency viruses isolated from rhesus macaques (SIVmac) contain an open reading frame (ORF), vpr, which has a coding potential of 97 to 101 amino acid residues. In this study, a vpr ORF-encoded protein of approximately 11 kDa was identified, and anti-vpr antibodies were detected in rhesus macaques infected by SIVmac. These results provide clear evidence that the vpr ORF is a coding gene of SIVmac. The vpr protein, like the vpx protein which is encoded by another accessory gene of SIVmac, was also found to be associated with viral particles. This observation demonstrates that more than one accessory gene product can be present in the virions of this family of retroviruses and raises the possibility that the vpr protein may have a role in early part of the virus life cycle.

Original languageEnglish (US)
Pages (from-to)5688-5693
Number of pages6
JournalJournal of Virology
Volume64
Issue number11
StatePublished - 1990
Externally publishedYes

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
vpr Gene Products
virion
Virion
Open Reading Frames
open reading frames
Macaca mulatta
Genes
Proteins
Retroviridae
proteins
Life Cycle Stages
genes
Anti-Idiotypic Antibodies
Genome
Viruses
Amino Acids
life cycle (organisms)
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Yu, X. F., Matsuda, M., Essex, M., & Lee, T. H. (1990). Open reading frame vpr of simian immunodeficiency virus encodes a virion-associated protein. Journal of Virology, 64(11), 5688-5693.

Open reading frame vpr of simian immunodeficiency virus encodes a virion-associated protein. / Yu, Xiao Fang; Matsuda, Michiko; Essex, Max; Lee, Tun Hou.

In: Journal of Virology, Vol. 64, No. 11, 1990, p. 5688-5693.

Research output: Contribution to journalArticle

Yu, XF, Matsuda, M, Essex, M & Lee, TH 1990, 'Open reading frame vpr of simian immunodeficiency virus encodes a virion-associated protein', Journal of Virology, vol. 64, no. 11, pp. 5688-5693.
Yu, Xiao Fang ; Matsuda, Michiko ; Essex, Max ; Lee, Tun Hou. / Open reading frame vpr of simian immunodeficiency virus encodes a virion-associated protein. In: Journal of Virology. 1990 ; Vol. 64, No. 11. pp. 5688-5693.
@article{dd3c7a252f1147cd965ef787acd99799,
title = "Open reading frame vpr of simian immunodeficiency virus encodes a virion-associated protein",
abstract = "The genomes of simian immunodeficiency viruses isolated from rhesus macaques (SIVmac) contain an open reading frame (ORF), vpr, which has a coding potential of 97 to 101 amino acid residues. In this study, a vpr ORF-encoded protein of approximately 11 kDa was identified, and anti-vpr antibodies were detected in rhesus macaques infected by SIVmac. These results provide clear evidence that the vpr ORF is a coding gene of SIVmac. The vpr protein, like the vpx protein which is encoded by another accessory gene of SIVmac, was also found to be associated with viral particles. This observation demonstrates that more than one accessory gene product can be present in the virions of this family of retroviruses and raises the possibility that the vpr protein may have a role in early part of the virus life cycle.",
author = "Yu, {Xiao Fang} and Michiko Matsuda and Max Essex and Lee, {Tun Hou}",
year = "1990",
language = "English (US)",
volume = "64",
pages = "5688--5693",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Open reading frame vpr of simian immunodeficiency virus encodes a virion-associated protein

AU - Yu, Xiao Fang

AU - Matsuda, Michiko

AU - Essex, Max

AU - Lee, Tun Hou

PY - 1990

Y1 - 1990

N2 - The genomes of simian immunodeficiency viruses isolated from rhesus macaques (SIVmac) contain an open reading frame (ORF), vpr, which has a coding potential of 97 to 101 amino acid residues. In this study, a vpr ORF-encoded protein of approximately 11 kDa was identified, and anti-vpr antibodies were detected in rhesus macaques infected by SIVmac. These results provide clear evidence that the vpr ORF is a coding gene of SIVmac. The vpr protein, like the vpx protein which is encoded by another accessory gene of SIVmac, was also found to be associated with viral particles. This observation demonstrates that more than one accessory gene product can be present in the virions of this family of retroviruses and raises the possibility that the vpr protein may have a role in early part of the virus life cycle.

AB - The genomes of simian immunodeficiency viruses isolated from rhesus macaques (SIVmac) contain an open reading frame (ORF), vpr, which has a coding potential of 97 to 101 amino acid residues. In this study, a vpr ORF-encoded protein of approximately 11 kDa was identified, and anti-vpr antibodies were detected in rhesus macaques infected by SIVmac. These results provide clear evidence that the vpr ORF is a coding gene of SIVmac. The vpr protein, like the vpx protein which is encoded by another accessory gene of SIVmac, was also found to be associated with viral particles. This observation demonstrates that more than one accessory gene product can be present in the virions of this family of retroviruses and raises the possibility that the vpr protein may have a role in early part of the virus life cycle.

UR - http://www.scopus.com/inward/record.url?scp=0024990404&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024990404&partnerID=8YFLogxK

M3 - Article

C2 - 2145446

AN - SCOPUS:0024990404

VL - 64

SP - 5688

EP - 5693

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 11

ER -