Oogenesis and programmed cell death of nurse cells in the endoparasitoid, Pteromalus puparum

Shengzhang Dong, Gong Yin Ye, Jian Yang Guo, Xiao Ping Yu, Cui Hu

Research output: Contribution to journalArticle

Abstract

In this study, we describe the features of oogenesis in the endoparasitoid, Pteromalus puparum, as well as the distinct type of programmed cell death of the nurse cells through conventional light and fluorescent markers for apoptosis and immunofluorescent analysis. Oogenesis in this endoparasitoid is divided into five stages, of which stages 1-2, 3-4, and 5 are corresponding to previtellogenic growth, yolk uptake, and the formation of egg envelopes, respectively. From these studies, we demonstrate two critical events, which are vitellogenin absorption and rapid transfer of nurse cell content, resulting in remarkable increase in the volume of oocytes during oogenesis in this endoparasitoid. Vitellogenin absorption initiates in the oocyte of early stage 3, and bulk transfer of nurse cell content into the oocyte occurs at stage 4 of oogenesis in P. puparum, which is mainly characterized with the programmed cell death in the nurse cell complex.

Original languageEnglish (US)
Pages (from-to)673-680
Number of pages8
JournalMicroscopy Research and Technique
Volume73
Issue number7
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

Fingerprint

Oogenesis
apoptosis
Cell death
gametocytes
Cell Death
Nurses
Oocytes
Vitellogenins
cells
eggs
markers
Ovum
envelopes
Apoptosis
Light
Growth

Keywords

  • Nurse cell
  • Oogenesis
  • Programmed cell death
  • Pteromalus puparum

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

Cite this

Oogenesis and programmed cell death of nurse cells in the endoparasitoid, Pteromalus puparum. / Dong, Shengzhang; Ye, Gong Yin; Guo, Jian Yang; Yu, Xiao Ping; Hu, Cui.

In: Microscopy Research and Technique, Vol. 73, No. 7, 01.07.2010, p. 673-680.

Research output: Contribution to journalArticle

Dong, Shengzhang ; Ye, Gong Yin ; Guo, Jian Yang ; Yu, Xiao Ping ; Hu, Cui. / Oogenesis and programmed cell death of nurse cells in the endoparasitoid, Pteromalus puparum. In: Microscopy Research and Technique. 2010 ; Vol. 73, No. 7. pp. 673-680.
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