Ontogeny of type 1 angiotensin II receptor gene expression in the rat

Alda Tufro-McReddie, Jeffrey K. Harrison, Allen D Everett, R. Ariel Gomez

Research output: Contribution to journalArticle

Abstract

To determine whether the expression of the type 1 angiotensin II receptor (AT1) gene is developmentally regulated and whether the regulation is tissue specific, AT1 mRNA levels were determined by Northern blot analysis in livers and kidneys from fetal, newborn, and adult rats, using a 1133-bp rat AT1 cDNA. In the liver, AT1 mRNA levels increased fivefold from 15 d gestation to 5 d of age. Liver AT1 mRNA levels at 5 d of age were similar to those of adult rats. In the kidney, AT1 mRNA levels were higher in immature than in adult animals. The intrarenal distribution of AT1 mRNA was assessed by in situ hybridization to a 35S-labeled 24 residues oligonucleotide complementary to rat AT1 mRNA. In the adult, AT1 mRNA was present in glomeruli, arteries, and vasa recta, whereas in the newborn AT1 mRNA was observed also over the nephrogenic area of the cortex. We conclude that: (a) fetal kidney and liver express the AT1 gene; (b) the AT1 gene expression is developmentally regulated in a tissue-specific manner; (c) during maturation, localization of AT1 mRNA in the kidney shifts from a widespread distribution in the nephrogenic cortex to specific sites in glomeruli, arteries, and vasa recta, suggesting a role for the angiotensin receptor in nephron growth and development.

Original languageEnglish (US)
Pages (from-to)530-537
Number of pages8
JournalJournal of Clinical Investigation
Volume91
Issue number2
StatePublished - Feb 1993
Externally publishedYes

Fingerprint

Angiotensin Type 1 Receptor
Gene Expression
Messenger RNA
Kidney
Liver
Rectum
Arteries
Angiotensin Receptors
Nephrons
Growth and Development
Oligonucleotides
Angiotensin II
Northern Blotting
Genes
In Situ Hybridization
Complementary DNA
Pregnancy

Keywords

  • Development
  • In situ hybridization
  • Kidney
  • Liver
  • mRNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tufro-McReddie, A., Harrison, J. K., Everett, A. D., & Gomez, R. A. (1993). Ontogeny of type 1 angiotensin II receptor gene expression in the rat. Journal of Clinical Investigation, 91(2), 530-537.

Ontogeny of type 1 angiotensin II receptor gene expression in the rat. / Tufro-McReddie, Alda; Harrison, Jeffrey K.; Everett, Allen D; Gomez, R. Ariel.

In: Journal of Clinical Investigation, Vol. 91, No. 2, 02.1993, p. 530-537.

Research output: Contribution to journalArticle

Tufro-McReddie, A, Harrison, JK, Everett, AD & Gomez, RA 1993, 'Ontogeny of type 1 angiotensin II receptor gene expression in the rat', Journal of Clinical Investigation, vol. 91, no. 2, pp. 530-537.
Tufro-McReddie, Alda ; Harrison, Jeffrey K. ; Everett, Allen D ; Gomez, R. Ariel. / Ontogeny of type 1 angiotensin II receptor gene expression in the rat. In: Journal of Clinical Investigation. 1993 ; Vol. 91, No. 2. pp. 530-537.
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