The ontogeny of dopamine transporter (DAT) gene expression was studied in the rat brain. DAT mRNA was first detected in neurons of the ventrocaudal mesencephalon on embryonic day 14 (E14). By E18, intensely expressing neurons in the ventral tegmental area and substantia nigra resembled the pattern found in adult midbrain. DAT mRNA is not abundant in the hypothalamus and the olfactory bulb at any stage of development. These results parallel those noted for tyrosine hydroxylase gene expression in mesencephalon.
- Dopamine transporter
- In situ hybridization histochemistry
- Substantia nigra
- Ventral tegmental area
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience