One-step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples

F. Nato, A. Boutonnier, M. Rajerison, P. Grosjean, S. Dartevelle, A. Guénolé, N. A. Bhuiyan, D. A. Sack, G. B. Nair, J. M. Fournier, S. Chanteau

Research output: Contribution to journalArticle

Abstract

We describe the development and evaluation of a rapid diagnostic test for Vibrio cholerae O1 and O139 based on lipopolysaccharide detection using gold particles. The specificity ranged between 84 and 100%. The sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in Madagascar and Bangladesh. The dipstick can provide a simple tool for epidemiological surveys.

Original languageEnglish (US)
Pages (from-to)476-478
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume10
Issue number3
DOIs
StatePublished - Jun 2003
Externally publishedYes

Fingerprint

Vibrio cholerae O139
Vibrio cholerae O1
Madagascar
Bangladesh
Routine Diagnostic Tests
Gold
Lipopolysaccharides
Surveys and Questionnaires

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

One-step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples. / Nato, F.; Boutonnier, A.; Rajerison, M.; Grosjean, P.; Dartevelle, S.; Guénolé, A.; Bhuiyan, N. A.; Sack, D. A.; Nair, G. B.; Fournier, J. M.; Chanteau, S.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 10, No. 3, 06.2003, p. 476-478.

Research output: Contribution to journalArticle

Nato, F, Boutonnier, A, Rajerison, M, Grosjean, P, Dartevelle, S, Guénolé, A, Bhuiyan, NA, Sack, DA, Nair, GB, Fournier, JM & Chanteau, S 2003, 'One-step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples', Clinical and Diagnostic Laboratory Immunology, vol. 10, no. 3, pp. 476-478. https://doi.org/10.1128/CDLI.10.3.476-478.2003
Nato, F. ; Boutonnier, A. ; Rajerison, M. ; Grosjean, P. ; Dartevelle, S. ; Guénolé, A. ; Bhuiyan, N. A. ; Sack, D. A. ; Nair, G. B. ; Fournier, J. M. ; Chanteau, S. / One-step immunochromatographic dipstick tests for rapid detection of Vibrio cholerae O1 and O139 in stool samples. In: Clinical and Diagnostic Laboratory Immunology. 2003 ; Vol. 10, No. 3. pp. 476-478.
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