One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

Daniel G. Gibson, Gwynedd A. Benders, Kevin C. Axelrod, Jayshree Zaveri, Mikkel A. Algire, Monzia Moodie, Michael G. Montague, J. Craig Venter, Hamilton O. Smith, Clyde A. Hutchison

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Medicine & Life Sciences