One polypeptide with two aminoacyl-tRNA synthetase activities

Constantinos Stathopoulos, Tong Li, Randy Longman, Ute C. Vothknecht, Hubert D. Becker, Michael Ibba, Dieter Söll

Research output: Contribution to journalArticle

Abstract

The genome sequences of certain archaea do not contain recognizable cysteinyl-transfer RNA (tRNA) synthetases, which are essential for messenger RNA-encoded protein synthesis. However, a single cysteinyl-tRNA synthetase activity was detected and purified from one such organism, Methanococcus jannaschii. The amino-terminal sequence of this protein corresponded to the predicted sequence of prolyl-tRNA synthetase. Biochemical and genetic analyses indicated that this archaeal form of prolyl-tRNA synthetase can synthesize both cysteinyl-tRNA(Cys) and prolyl-tRNA(Pro). The ability of one enzyme to provide two aminoacyl-tRNAs for protein synthesis raises questions about concepts of substrate specificity in protein synthesis and may provide insights into the evolutionary origins of this process.

Original languageEnglish (US)
Pages (from-to)479-482
Number of pages4
JournalScience
Volume287
Issue number5452
DOIs
StatePublished - Jan 21 2000
Externally publishedYes

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Amino Acyl-tRNA Synthetases
Transfer RNA
Peptides
Proteins
Methanocaldococcus
Archaea
Substrate Specificity
Molecular Biology
Genome
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Stathopoulos, C., Li, T., Longman, R., Vothknecht, U. C., Becker, H. D., Ibba, M., & Söll, D. (2000). One polypeptide with two aminoacyl-tRNA synthetase activities. Science, 287(5452), 479-482. https://doi.org/10.1126/science.287.5452.479

One polypeptide with two aminoacyl-tRNA synthetase activities. / Stathopoulos, Constantinos; Li, Tong; Longman, Randy; Vothknecht, Ute C.; Becker, Hubert D.; Ibba, Michael; Söll, Dieter.

In: Science, Vol. 287, No. 5452, 21.01.2000, p. 479-482.

Research output: Contribution to journalArticle

Stathopoulos, C, Li, T, Longman, R, Vothknecht, UC, Becker, HD, Ibba, M & Söll, D 2000, 'One polypeptide with two aminoacyl-tRNA synthetase activities', Science, vol. 287, no. 5452, pp. 479-482. https://doi.org/10.1126/science.287.5452.479
Stathopoulos C, Li T, Longman R, Vothknecht UC, Becker HD, Ibba M et al. One polypeptide with two aminoacyl-tRNA synthetase activities. Science. 2000 Jan 21;287(5452):479-482. https://doi.org/10.1126/science.287.5452.479
Stathopoulos, Constantinos ; Li, Tong ; Longman, Randy ; Vothknecht, Ute C. ; Becker, Hubert D. ; Ibba, Michael ; Söll, Dieter. / One polypeptide with two aminoacyl-tRNA synthetase activities. In: Science. 2000 ; Vol. 287, No. 5452. pp. 479-482.
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