One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE)

Julie L. Brunelle, Rachel Green

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part C
PublisherAcademic Press Inc.
Pages151-159
Number of pages9
ISBN (Print)9780124201194
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume541
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

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Keywords

  • Ammonium persulfate (APS)
  • One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)
  • Polyacrylamide
  • Polyacrylamide gel electrophoresis
  • SDS-PAGE gel
  • Stacking gel
  • Tetramethylethylenediamine (TEMED)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Brunelle, J. L., & Green, R. (2014). One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE). In Laboratory Methods in Enzymology: Protein Part C (pp. 151-159). (Methods in Enzymology; Vol. 541). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420119-4.00012-4