One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE)

Julie L. Brunelle, Rachel Green

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages151-159
Number of pages9
Volume541
ISBN (Print)9780124201194
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume541
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Electrophoresis
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Proteins
Purification
Molecular Weight
Molecular weight
Molecules
polyacrylamide gels

Keywords

  • Ammonium persulfate (APS)
  • One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)
  • Polyacrylamide
  • Polyacrylamide gel electrophoresis
  • SDS-PAGE gel
  • Stacking gel
  • Tetramethylethylenediamine (TEMED)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Brunelle, J. L., & Green, R. (2014). One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE). In Methods in Enzymology (Vol. 541, pp. 151-159). (Methods in Enzymology; Vol. 541). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420119-4.00012-4

One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE). / Brunelle, Julie L.; Green, Rachel.

Methods in Enzymology. Vol. 541 Academic Press Inc., 2014. p. 151-159 (Methods in Enzymology; Vol. 541).

Research output: Chapter in Book/Report/Conference proceedingChapter

Brunelle, JL & Green, R 2014, One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE). in Methods in Enzymology. vol. 541, Methods in Enzymology, vol. 541, Academic Press Inc., pp. 151-159. https://doi.org/10.1016/B978-0-12-420119-4.00012-4
Brunelle JL, Green R. One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE). In Methods in Enzymology. Vol. 541. Academic Press Inc. 2014. p. 151-159. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-420119-4.00012-4
Brunelle, Julie L. ; Green, Rachel. / One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE). Methods in Enzymology. Vol. 541 Academic Press Inc., 2014. pp. 151-159 (Methods in Enzymology).
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